Molecular Characterization of Helicobacter Pylori in Human and Pet Animal in Egypt (Ismailia Governorate)
Ibrahim, H., Eid, H., Enany, S., Kelany, M. (2021). Molecular Characterization of Helicobacter Pylori in Human and Pet Animal in Egypt (Ismailia Governorate). EKB Journal Management System, 26(2), 287-302. doi: 10.21608/scvmj.2021.217826
Heba Ibrahim; Hamza Eid; Shymaa Enany; Mahmoud Kelany. "Molecular Characterization of Helicobacter Pylori in Human and Pet Animal in Egypt (Ismailia Governorate)". EKB Journal Management System, 26, 2, 2021, 287-302. doi: 10.21608/scvmj.2021.217826
Ibrahim, H., Eid, H., Enany, S., Kelany, M. (2021). 'Molecular Characterization of Helicobacter Pylori in Human and Pet Animal in Egypt (Ismailia Governorate)', EKB Journal Management System, 26(2), pp. 287-302. doi: 10.21608/scvmj.2021.217826
Ibrahim, H., Eid, H., Enany, S., Kelany, M. Molecular Characterization of Helicobacter Pylori in Human and Pet Animal in Egypt (Ismailia Governorate). EKB Journal Management System, 2021; 26(2): 287-302. doi: 10.21608/scvmj.2021.217826

Molecular Characterization of Helicobacter Pylori in Human and Pet Animal in Egypt (Ismailia Governorate)

Suez Canal Veterinary Medical Journal. SCVMJ
Article 3, Volume 26, Issue 2, December 2021, Page 287-302  XML
Document Type: Original Article
DOI: 10.21608/scvmj.2021.217826
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Authors
Heba Ibrahim* 1; Hamza Eid2; Shymaa Enany3; Mahmoud Kelany4
1Al-Mokhtabar laboratories, Suez
2Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal university.
3Microbiology and Immunology Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
4Microbiology Department at National Institute of Oceanography and Fisheries, Suez, Egypt (NIOF).
Abstract
The genus Helicobacter contains more than 35 species, Gram- negative microaerophilic bacteria, which colonizes the gastrointestinal tracts of humans and different animals. aim of work transfer to end of introduction to determine the susceptibility of H. pylori isolates to several antimicrobial agents to overcome the H. pylori treatment failure. For this purpose, 25stool sample from dog, 20 stool samples from cat and 75 stool samples from their human owners were collected for H. pylori infection. H. pylori was detected by culture in 36%, 20% and 41.3% of dog, Cat and human samples, respectively. Antimicrobial resistance of H. pylori in human, dog and cat to ceftazidime is100% .to amoxicillin is 76%, 100% and 100% respectively, to bacitracin is 88%, 100% and 100% respectively, to erythromycin is 80%, 100% and 100% respectively, to trimethoprim sulphate is 48%, 50% and 50% respectively, to doxycycline is 40%, 50% and 50% respectively, to levofloxacin is 24%, 50% and 50% respectively, to nitrofurantoin is 44%, 50% and 50% respectively. 9 stool samples were collected from human, dog and cat examined for presence of specific16s rRNA gene for H. pylori by nested PCR. The positive samples were subjected to Helicobacter genus- specific 16s r RNA gene sequencing.
Keywords
H. pylori; Antimicrobial resistance; 16s r RNA sequencing
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