IN VITRO STUDIES ON TARO, Colocasia esculenta var. esculenta (L) Schott. | ||||
| Journal of Plant Production | ||||
| Article 18, Volume 32, Issue 8, August 2007, Page 6631-6642 PDF (504.36 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2007.220206 | ||||
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| Author | ||||
| M. S.S. El-Shabasi, | ||||
| Hort. Res. Inst., Agric. Res. Centre, Giza, Egypt. | ||||
| Abstract | ||||
| An effective method (protocol) for micropropagation of taro (Colocasia esculenta var. esculenta cv. Egyptian) was developed. This method comprised of four successive stages. In stage I "callus induction", the results showed that the highest percentage of callus induction was obtained by culturing the axillary buds explants on AZ-medium and on both half and full strength of MS-medium. Supplementation of MS-medium with 0.45 mg/l dicamba (potent auxin) obviously encouraged callus induction percentage comparing to the other concentrations. In stage II "callus growth and shoot formation", the highest percentage of shoot formation was obtained when the initiated calli were subcultured onto MS-medium supplemented with 0.4 mg/l dimethylallylamino purine (2iP) + 0.45 mg/l dicamba. The highest percentage of green meristematic regions production was obtained in case of adding 0.1 mg/l 2iP + 0.45 mg/l dicamba to the culture medium. Also, in this stage, the highest percentage of plantlets formation (70%) was obtained by culluring the initiated calli on MS-medium containing 0.60 mg/l thidiazuron (TDZ) alone. Supplementation of MS-medium with 1.05 mg/l TDZ gave the highest number of shoots per responded cultures. In stage III " plantlet formation and elongation", the obtained friable callus alone or along with green meristematic regions which derived in the previously stage were used as plant material. When they subcultured on MS-medium supplemented with 3.0 mg/l kinetin + 0.3 mg/l IAA + 20 mg/l adenine hemisulfate, the highest percentage of clusters of taro plantlets (77.5%) was obtained. In stage IV "acclimatization", plantlets derived in vitro with well developed roots were successfully acclimatized when potted in a peat moos – vermiculite (1:1 w/w) mixture. | ||||
| Keywords | ||||
| Taro; Colocasia esculenta var. esculenta (L) Schott; Araceae family; callus culture; micropropagation; tissue culture | ||||
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