'pEFFECT OF MEDIUM COMPOSITION ON THE HYPERHYDRICITY OF GLOBE ARTICHOKE IN VITRO | ||||
| Journal of Plant Production | ||||
| Article 25, Volume 32, Issue 8, August 2007, Page 6735-6743 PDF (480.27 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2007.220243 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| M. S.S. El-Shabasi, | ||||
| Hort. Res. Inst., Agric. Res. Centre, Giza, Egypt. | ||||
| Abstract | ||||
| Hyperhydricity or vitrescence was a frequent problem during the micropropagation of globe artichoke and it was influenced by medium composition, and type and concentration of cytokinins used in culture medium. Using a multiplication medium containing half-strength of NH4NO3 AND KNO3 and doubly weights of calcium chloride and magnesium sulphate specified for Murashige and Skoog (MS) medium recorded the lowest value of hyperhydric cultures percentage. In addition, this medium caused significant increases in leaves dry matter % as well as chlorophyll (a) and (b) content. Supplementation of MS medium with 2.0 mg/l kinetin + 1.0 mg/l IAA was the optimum for shoot proliferation with relatively low hyperhydricity | ||||
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