The deterrent effect of acetylcysteine against hepatic and renal damage in thiamethoxam exposed rats
Abdel-Razik, R., Mosallam, E., Hamed, N. (2022). The deterrent effect of acetylcysteine against hepatic and renal damage in thiamethoxam exposed rats. EKB Journal Management System, 65(9), 251-266. doi: 10.21608/ejchem.2022.114622.5209
Reda Khamis Abdel-Razik; Eman Mohammad Mosallam; Nadia Ali Hamed. "The deterrent effect of acetylcysteine against hepatic and renal damage in thiamethoxam exposed rats". EKB Journal Management System, 65, 9, 2022, 251-266. doi: 10.21608/ejchem.2022.114622.5209
Abdel-Razik, R., Mosallam, E., Hamed, N. (2022). 'The deterrent effect of acetylcysteine against hepatic and renal damage in thiamethoxam exposed rats', EKB Journal Management System, 65(9), pp. 251-266. doi: 10.21608/ejchem.2022.114622.5209
Abdel-Razik, R., Mosallam, E., Hamed, N. The deterrent effect of acetylcysteine against hepatic and renal damage in thiamethoxam exposed rats. EKB Journal Management System, 2022; 65(9): 251-266. doi: 10.21608/ejchem.2022.114622.5209

The deterrent effect of acetylcysteine against hepatic and renal damage in thiamethoxam exposed rats

Egyptian Journal of Chemistry
Article 24, Volume 65, Issue 9, September 2022, Page 251-266  XML PDF (925.18 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.114622.5209
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Authors
Reda Khamis Abdel-Razik email orcid 1; Eman Mohammad Mosallam2; Nadia Ali Hamed2
1ammalian & Aquatic Toxicology Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, 21616 -El-Sabahia, Alexandria, Egypt
2Mammalia & Aquatic Toxicology Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, 21616 -El-Sabahia, Alexandria, Egypt
Abstract
The biochemical, oxidative DNA damage, and the histological alterations associated with thiamethoxam (MX) exposure, a second-generation neonicotinoid broadly used in Egyptian agriculture, were assessed. Also, the role of N- acetylcysteine (NAC), (150 mg/kg/day) on the adverse effect of MX was investigated. Rats were orally preserved with a sub-lethal dose (1/50 LD50) of MX at 31.26 mg/kg/day, five doses/week for 28 days. The MX exposure resulted in a significant decrease in rats' body weight, protein concentration of both serum and urine, sera superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities compared to control. In addition increase in sera creatinine, urea, bilirubin, alkaline phosphatase (ALP), and malondialdehyde (MDA) levels were observed. While the assessment of DNA damage revealed significant elevation in 8-hydroxy-2'-deoxyguanosine (8-OH-2DG) levels in both serum (428.9%) and urine (234.6 %) samples. The present findings were supported by microscopic observation of liver and kidney tissues. Evidently, thiamethoxam can damage liver and kidney functions impaired the DNA, and caused histoarchitecture lesions in rats at the tested sublethal dose. In addition, NAC supplementation significantly attenuated the MX DNA damaging effect to be 49.1% and 50.18% for serum and urine, respectively compared to the control which reflects its protective properties against MX-induced hepatic-nephrotoxicity.
Keywords
DNA damage; Thiamethoxam, Hepatotoxicity; Histopathological analysis; Lipid peroxidation; Nephrotoxicity; oxidative stress
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