Comparative characterization of carob pulp and seeds extracts: HPLC, antimicrobial, anti-inflammatory, and cytotoxic studies | ||||
| Egyptian Journal of Chemistry | ||||
| Article 26, Volume 65, Issue 10, October 2022, Page 279-284 PDF (320.37 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2022.116534.5265 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Alaadin E. El-Haddad   1; Abdallah M. Gendy 2; Mohamed M. Amin 3; Walaa A. Alshareef 4; Heba A. El Gizawy 5
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| 1faculty of pharmacy October 6 university | ||||
| 2Pharmacology and Toxicology Department, Faculty of Pharmacy, October 6 University, Giza, Egypt. | ||||
| 3Pharmacology Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Giza, Egypt | ||||
| 4Microbiology and Immunology Department, Faculty of Pharmacy, October 6 University, Giza, Egypt | ||||
| 5Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Giza, Egypt. | ||||
| Abstract | ||||
| HPLC profile, antimicrobial, cytotoxic, and anti-inflammatory activities of carob (Ceratonia siliqua L.) pulp and seeds extracts were investigated. Gallic acid was the main identified phenolic. Ellagic acid, rutin, quercetin, and kaempferol were also tentatively identified. Carob seeds extract exhibited antimicrobial activity against Pseudomonas aeruginosa (6.25 and 25 mg mL-1 for minimum inhibitory concentration and minimum bactericidal concentration: respectively). Carob extracts showed a weak dose-response inhibition of the lipopolysaccharide induced nitric oxide release in RAW macrophages. Carob extracts even in the high dose did not inhibit the cell growth either against MCF-7 or HT-29 cell lines. Carob pods could be used as a functional food or food additive based on these findings. | ||||
| Keywords | ||||
| Carob; phenolics; HPLC; cytotoxic; antimicrobial; anti-inflammatory | ||||
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