RAPD –PCR analysis of four coral reef fish species, genus cephalopholis (Family: serranidae) in the Red sea.
A.M., A., .H, K., A., A. (2014). RAPD –PCR analysis of four coral reef fish species, genus cephalopholis (Family: serranidae) in the Red sea.. EKB Journal Management System, 18(2), 125-141. doi: 10.21608/ejabf.2014.2212
Abied A.M.; Khalil .H; Abu El Regal A.. "RAPD –PCR analysis of four coral reef fish species, genus cephalopholis (Family: serranidae) in the Red sea.". EKB Journal Management System, 18, 2, 2014, 125-141. doi: 10.21608/ejabf.2014.2212
A.M., A., .H, K., A., A. (2014). 'RAPD –PCR analysis of four coral reef fish species, genus cephalopholis (Family: serranidae) in the Red sea.', EKB Journal Management System, 18(2), pp. 125-141. doi: 10.21608/ejabf.2014.2212
A.M., A., .H, K., A., A. RAPD –PCR analysis of four coral reef fish species, genus cephalopholis (Family: serranidae) in the Red sea.. EKB Journal Management System, 2014; 18(2): 125-141. doi: 10.21608/ejabf.2014.2212

RAPD –PCR analysis of four coral reef fish species, genus cephalopholis (Family: serranidae) in the Red sea.

Egyptian Journal of Aquatic Biology and Fisheries
Article 10, Volume 18, Issue 2, April 2014, Page 125-141  XML PDF (1.7 MB)
Document Type: Original Article
DOI: 10.21608/ejabf.2014.2212
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Authors
Abied A.M.1; Khalil .H1; Abu El Regal A.2
1Zoology Dept. Faculty of Science, Qena, South Valley University, Egypt
2Marine Science Department, Faculty of Science, Port Said University, Egypt.
Abstract
The genetic relationships between four species of groupers: Cephalopholis oligosticta, C. sexmaculata, C. hemistiktos and C. argus, common coral reef fish in the Red Sea of family Serranidae were studied by using Random Amplified
Polymorphic DNA (RAPD) technique. Twenty random primers were used. Each primer was individually tested with the four fish species. Amplification products were resolved on agarose gel electrophoresis and visualized under UV light, then
photographed. The number and size of amplified DNA fragments showed a wide range of variation. Tabulation and analysis of the data using the genetic similarity coefficient were carried out. The highest genetic similarity observed was between C. hemistiktos and C argus and the lowest was between C. oligosticta and C.sexmaculata. Dendrogram analysis revealed three clusters: first, comprises C.hemistiktos and C. argus; second, contains C. oligosticta and (C. hemistiktos and C.argus); the third incompasses C. sexmaculata and (C. oligosticta, C. hemistiktos and
C. argus).
Keywords
Cephalopholis; Serranidae; RAPD –PCR; genetic variation; Red Sea
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