Virulence Genes in Pseudomonas aeruginosa Clinical Isolates | ||||
| Minia Journal of Medical Research | ||||
| Article 43, Volume 30, Issue 3, July 2019, Page 211-213 PDF (232.07 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjmr.2022.221930 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mohammed S. Mohammed; Ebtisam S. Mohamed; Wedad M. Abdel-Rahim; Alyaa E. Abdelkader | ||||
| Department of Microbiology, El-Minia Faculty of Medicine | ||||
| Abstract | ||||
| Introduction: Pseudomonas aeruginosa (P. aeruginosa), which is a non-fermentative Gram the negative opportunistic pathogen is the leading cause of many infections including pneumonia, wound, and urinary tract infection. Aim of the work: To determine the ability of P. aeruginosa clinical isolates to produce biofilm. To detect expression of Lec A gene and biofilm development. Materials and methods: Sample Collection and Microbiological Processing: Samples were collected and sent directly to the microbiology laboratory of Medical Microbiology and Immunology Department, Faculty of Medicine, Minia University for bacteriological study. It was done in the period from April 2017 to December 2017. Pus from wound was collected by using sterile ordinary swab, then plates of Cetramide agar media were inoculated and incubated under aerobic conditions at 37°C and a Gram stained film was done. Results: Patients and bacterial isolates: This study was conducted at Minia University Hospitals during the period from April 2017 till December 2018. Two hundred and twenty two samples were collected from surgical wounds from outpatient clinic and inpatient surgery department. Out of 222 bacterial isolates, one hundred isolates of P. aeruginosa were detected. They showed growth on cetramide agar. They were citrate and oxidase positive. They were indole test negative, methyl red test negative and VP test negative. TSI test showed red butt and red slant. Conclusion: 1- The degree of biofilm formation differs between different P. aeurginosa strains. 2- Lec A gene has a significant role in biofilm formation. 3- Imipenem is highly effective against P. aeurginosa in vitro. Biofilm producing strains have higher resistance to antibiotics even in their planktonic form than nonbiofilm-producing strains. Recommendations: Large-scale studies on a large number of P. aeruginosa clinical isolates to study their abilities to form biofilm and its relation to Lec A gene and antibiotic resistance. | ||||
| Keywords | ||||
| Virulence Genes; Pseudomonas aeruginosa; biofilm | ||||
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