Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system
Abd-Allah, E., EL- Ghareeb, A., Hafez, O., Abd EL-Rahman, H. (2022). Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system. EKB Journal Management System, 65(11), 677-689. doi: 10.21608/ejchem.2022.120399.5400
Entsar R. Abd-Allah; Abd EL-Wahab EL- Ghareeb; Olfat Serag Hafez; Heba Ali Abd EL-Rahman. "Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system". EKB Journal Management System, 65, 11, 2022, 677-689. doi: 10.21608/ejchem.2022.120399.5400
Abd-Allah, E., EL- Ghareeb, A., Hafez, O., Abd EL-Rahman, H. (2022). 'Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system', EKB Journal Management System, 65(11), pp. 677-689. doi: 10.21608/ejchem.2022.120399.5400
Abd-Allah, E., EL- Ghareeb, A., Hafez, O., Abd EL-Rahman, H. Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system. EKB Journal Management System, 2022; 65(11): 677-689. doi: 10.21608/ejchem.2022.120399.5400

Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system

Egyptian Journal of Chemistry
Article 60, Volume 65, Issue 11, November 2022, Page 677-689  XML PDF (1.18 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.120399.5400
View on SCiNiTO View on SCiNiTO
Authors
Entsar R. Abd-Allah1; Abd EL-Wahab EL- Ghareeb2; Olfat Serag Hafez3; Heba Ali Abd EL-Rahman email 3
1Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Egypt
2Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt
3Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt
Abstract
Neonicotinoid insecticides usage is currently widespread, but this poses a challenge when considering the potential for occupational and environmental contamination. One of the most extensively used insecticides is thiamethoxam (TMX), a second-generation neonicotinoid insecticide. This study aimed to see if sub-lethal dosages of TMX insecticide had any negative impacts on epididymal sperm parameters, serum hormones, oxidative status, and testicular histology. The experimental cohorts were given a low dose of TMX (156 mg/kg bw), a high dose of TMX (312 mg/kg bw), or an untreated control for eight weeks. The sperm count, percent of viability, motility, and motility progressing and fructose level significantly decreased in both TMX-treated groups compared to the control group. Furthermore, TMX administration induced sperm morphological defects, serum hormone disturbances as significant reduction testosterone level, oxidant/antioxidant status imbalance as significant decline in catalase (CAT) and glutagthione peroxidase (GSH) content and significant rise in malondialdehyde (MDA) level and testicular histopathological alternation. TMX caused significant increase DNA damage in testicular tissue represented in tail DNA percent, comet percent, comet length, tail moment, and Olive moment. In conclusion, TMX exposure may have a deleterious impact on male albino rats' fertility through spermatogenesis, steroidogenesis and testicular redox status disruption, and testicles DNA impairment.
Keywords
Thiamethoxam; sperm quality; histopathology; reproductive hormones; oxidative stress; DNA damage; male Wistar rats
Statistics
Article View: 491
PDF Download: 280