Vitamin D3 and Alpha-Lipoic Acid alleviate chronic immobilization stress induced metabolic changes in adult male rats

Bahaa El-Dein, Ienass; Ahmed, Mona Ahmed; Mohamed, Fatma Ahmed; Soliman, Nevine Bahaa; Lasheen, Noha; Abou-Bakr, Doaa

doi:10.21608/besps.2021.102328.1112

	Vitamin D3 and Alpha-Lipoic Acid alleviate chronic immobilization stress induced metabolic changes in adult male rats
Bulletin of Egyptian Society for Physiological Sciences
Article 4, Volume 42, Issue 2, April 2022, Page 180-201 PDF (593.17 K)
Document Type: Original Article
DOI: 10.21608/besps.2021.102328.1112
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Authors
Ienass Bahaa El-Dein¹; Mona Ahmed Ahmed ²; Fatma Ahmed Mohamed³; Nevine Bahaa Soliman⁴; Noha Lasheen ¹; Doaa Abou-Bakr ⁵
¹physiology department, faculty of medicine, ain shams university, Cairo, Egypt
²Physiology department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
³Physiology department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
⁴Histology department, Faculty of medicine, Ain shams university, Cairo, Egypt
⁵Physiology department, Faculty of medicine, Ain shams university, Cairo, Egypt
Abstract
Abstract: Context: Stress disturbs metabolic processes. Vitamin-D3 and alpha-lipoic-acid (ALA) are known by their bone mineralizing and antioxidant effect respectively, with some metabolic effects. Objective: Elucidate the effect of Vitamin-D3 and ALA on metabolic responses to chronic stress and clarify their underlying mechanism(s). Materials and Methods: 40 adult local strain albino male rats were randomly allocated into Control, Stressed, Vitamin-D3- and ALA-treated stressed groups. Rats were subjected to determination of anthropometric, glycemic parameters, lipid profile, Malondialdehyde (MDA), total antioxidant capacity (TAC), nitrites, insulin, leptin, testosterone, estrogen. Also, histopathological study and immunohistochemistry for caspase-3 of pancreatic tissue were performed. Results: Upon vitamin-D3 and ALA treatment, fasting plasma glucose and glucose output by kidneys, lipid profile, estrogen and caspase-3 area% were reduced, while glucose uptake by diaphragm, HOMA-B and testosterone were elevated when comparted with stressed group. Only ALA showed significantly elevated TAC and insulin when compared with stressed rats. Insignificant changes were shown between the treated groups, except for reduced testosterone and elevated estrogen in ALA-treated. Conclusion: Both treatments are effective in amelioration of glycemic and lipidemic derangements induced by chronic stress by restoration of normal sex hormones pattern, antioxidant, more prominent with ALA, and antiapoptotic effects.
Keywords
Vitamin-D3; alpha-lipoic-acid; chronic immobilization stress; sex hormones; antiapoptotic


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