Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats
Adam, G. (2022). Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats. EKB Journal Management System, 7(2), 28-33. doi: 10.21608/javs.2022.119861.1123
Gareeballah Osman Adam. "Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats". EKB Journal Management System, 7, 2, 2022, 28-33. doi: 10.21608/javs.2022.119861.1123
Adam, G. (2022). 'Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats', EKB Journal Management System, 7(2), pp. 28-33. doi: 10.21608/javs.2022.119861.1123
Adam, G. Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats. EKB Journal Management System, 2022; 7(2): 28-33. doi: 10.21608/javs.2022.119861.1123

Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats

Journal of Applied Veterinary Sciences
Article 5, Volume 7, Issue 2, April 2022, Page 28-33  XML PDF (625.99 K)
Document Type: Original Article
DOI: 10.21608/javs.2022.119861.1123
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Author
Gareeballah Osman Adam email orcid
Assistant Professor at Department of Pharmacology and Toxicology, College of Veterinary Medicine, Sudan University of Science and Technology, Sudan.
Abstract
Experiments on pulmonary pharmacology are often performed in laboratory animals. Investigators collect organs, samples, or record modifications in the respiratory system during anaesthesia, which might significantly influence the data attained. Indeed,  it is recommended to cautiously select the anaesthetic adapted for lung studies. This experiment aimed to evaluate three selected anaesthetics usually used in laboratory animal experiments, namely, (Zolite-rumpon (Z+R) mixture, halothane (HAL), and urethane (URE)) in rats. Rats were divided into four groups, control group, kept without treatment; Zolitel and Rumpon (Z+R) at a dose of 5  and  30  mg/kg body weight intraperitoneally, respectively; HAL group (5% with oxygen supply), and URE (1.5 mg/kg body weight) by inhalation. Blood samples were collected to determine the pH of the blood, bicarbonate (HCO3-), carbon dioxide partial pressure (pCO2), oxygen partial pressure (pO2), alveolar oxygen (A), saturated oxygen (O2 Sat), magnesium  (Mg2+), calcium (Ca2+), sodium (Na+), potassium (K+), and chloride (Cl−) ions, haematocrit (Hct), and haemoglobin (Hb). The result indicates that the Z+R mixture causes slight to no differences in the measured data. At the same time, HAL and URE greatly reduced the blood pH and significantly disrupted the values of  HCO3-, pCO2, pO2, A, and O2 Sat. Together, HAL and URE induced metabolic acidosis and respiratory depression. Hence, the Z+R mix is suitable for the three anaesthetics pulmonary pharmacology experiments.
Keywords
Anesthetic; Blood gases; Pulmonary; Zolitel-Rumpon
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