8-hydroxy-2'-deoxyguanosine and TP53 in Egyptian Patients with Hepatitis C Viral Chronic Liver Diseases: Insight into the Pathogenesis and Predictive Force
El-Emshaty, H., Osman, S., El-Taweel, F., El-Hemaly, M., Ismail, H. (2022). 8-hydroxy-2'-deoxyguanosine and TP53 in Egyptian Patients with Hepatitis C Viral Chronic Liver Diseases: Insight into the Pathogenesis and Predictive Force. EKB Journal Management System, 8(1), 46-56. doi: 10.21608/jbaar.2022.223518
Hoda El-Emshaty; Somaia M. Osman; Fathy M. El-Taweel; Mohamed M. El-Hemaly; Hisham Ismail. "8-hydroxy-2'-deoxyguanosine and TP53 in Egyptian Patients with Hepatitis C Viral Chronic Liver Diseases: Insight into the Pathogenesis and Predictive Force". EKB Journal Management System, 8, 1, 2022, 46-56. doi: 10.21608/jbaar.2022.223518
El-Emshaty, H., Osman, S., El-Taweel, F., El-Hemaly, M., Ismail, H. (2022). '8-hydroxy-2'-deoxyguanosine and TP53 in Egyptian Patients with Hepatitis C Viral Chronic Liver Diseases: Insight into the Pathogenesis and Predictive Force', EKB Journal Management System, 8(1), pp. 46-56. doi: 10.21608/jbaar.2022.223518
El-Emshaty, H., Osman, S., El-Taweel, F., El-Hemaly, M., Ismail, H. 8-hydroxy-2'-deoxyguanosine and TP53 in Egyptian Patients with Hepatitis C Viral Chronic Liver Diseases: Insight into the Pathogenesis and Predictive Force. EKB Journal Management System, 2022; 8(1): 46-56. doi: 10.21608/jbaar.2022.223518

8-hydroxy-2'-deoxyguanosine and TP53 in Egyptian Patients with Hepatitis C Viral Chronic Liver Diseases: Insight into the Pathogenesis and Predictive Force

Journal of Bioscience and Applied Research
Article 3, Volume 8, Issue 1, March 2022, Page 46-56  XML PDF (529.44 K)
Document Type: Original Article
DOI: 10.21608/jbaar.2022.223518
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Authors
Hoda El-Emshaty email 1; Somaia M. Osman2; Fathy M. El-Taweel2; Mohamed M. El-Hemaly1; Hisham Ismail3
1Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt
2Chemistry Dept., Faculty of Science, Damietta University, New Damietta, Egypt
3Biochemistry Division, Chemistry Dept., Faculty of Science, Minia University, Minia, Egypt
Abstract
Reactive oxygen species (ROS) is excessively generated during tumor development yielding the oxidatively modified products of proteins and DNA. These DNA alterations could contribute to the development of cancer through the activation of oncogenes and inactivating tumor suppressor genes (TSGs). Therefore, 8-OHdG DNA oxidative damage and TP53 protein expression were evaluated amongst HCV-Chronic liver disease patients to explore their possible role in hepatocarcinogenesis and to predict HCC development at early stages. A total of 141 patients with HCV-related liver diseases; 69 with hepatocellular carcinoma and 72 with liver cirrhosis were enrolled in this study in addition to 56 healthy subjects.Serum 8-OHdG and TP53 expression by ELISA were markedly elevated in HCC patients compared to LC and healthy individuals (p < 0.0001). A significant correlation was noted for 8-OHdG and TP53 with disease progression and tumor differentiation but not with tumor site. 8-OHdG and TP53 were highly (p < 0.05) predicting for HCC at early stages and the diagnostic performance for discriminating HCC from LC by ROC curve showed the best AUC was recorded for 8-OHdG (0.745) followed by TP53 (0.667) with accuracy (87.2% and 82% respectively). Therefore, HCV-induced oxidative DNA damage could increase the carcinogenic potential of HCC development through the activation of TP53. 
Keywords
8-OHdG; TP53 Ag; ELISA; HCV; Hepatocellular carcinoma
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