MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE ‎‎EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN ‎BUFFALOES
Ali, S., elsaudi, A., Saeed, A., Abushady, A. (2022). MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE ‎‎EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN ‎BUFFALOES. EKB Journal Management System, 59(1), 9-18. doi: 10.21608/ejap.2022.114631.1032
sarah Gamal Ali; alia Ahmed elsaudi; Ayman Moustafa Saeed; Asmaa Mohammed Abushady. "MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE ‎‎EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN ‎BUFFALOES". EKB Journal Management System, 59, 1, 2022, 9-18. doi: 10.21608/ejap.2022.114631.1032
Ali, S., elsaudi, A., Saeed, A., Abushady, A. (2022). 'MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE ‎‎EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN ‎BUFFALOES', EKB Journal Management System, 59(1), pp. 9-18. doi: 10.21608/ejap.2022.114631.1032
Ali, S., elsaudi, A., Saeed, A., Abushady, A. MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE ‎‎EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN ‎BUFFALOES. EKB Journal Management System, 2022; 59(1): 9-18. doi: 10.21608/ejap.2022.114631.1032

MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE ‎‎EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN ‎BUFFALOES

Egyptian Journal of Animal Production
Article 2, Volume 59, Issue 1, February 2022, Page 9-18  XML PDF (1.54 MB)
Document Type: Original Article
DOI: 10.21608/ejap.2022.114631.1032
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Authors
sarah Gamal Ali email 1; alia Ahmed elsaudi2; Ayman Moustafa Saeed3; Asmaa Mohammed Abushady4
1department of biotechnology, animal production research institute
2Department of Genetics, Faculty of Agriculture, Ain shams University, Soubra El- Khaymah, Cairo, Egypt.
3Department of Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt.
4Biotechnology school, Nile University, Sheikh Zayed, Giza, Egypt. Department of Genetics, Faculty of Agriculture, Ain shams University, Soubra El- Khaymah, Cairo, Egypt.
Abstract
The goal of this work was to describe the sequences of some candidate genes (IGF-I, IGF-I receptor, and Leptin) that are associated with economically important quantitative aspects in dairy buffalo, such as reproductive and productive attributes, as well as milk composition. Ninety-nine dairy buffaloes were used to compare the pure Egyptian buffalo (PE) with the Egyptian–Italian crossbred G1 (25.0%), G2 (50.0%), G3 (62.5%), G4 (75.0%), G5 (87.5%), and G6 (94.0%), respectively. All buffaloes investigated were genotyped BB, which means they were negative for the SnaBI at position 224^225 (TAC^GTA) of the IGF-I regulatory region, and they were genotyped AA-positive for the IGF-I receptor TaqI at position 47^48 (T^CGA). They also tested positive for the leptin gene's Alu1 restriction site yielding three products with genotype GT that was 55-, 118-, and 205-bp in length (AG^CT). Finally, the PE and Egyptian-Italian crossbred demonstrate monomorphism since the two Bubaline populations are closely related and the genes in question are maintained. More research is needed to learn more about Egyptian-Italian buffalo crossbreeds before national crossbreeding initiatives may be expanded.
Keywords
Egyptian-Italian buffalo; insulin-like growth factor; leptin; restriction fragment length polymorphism
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