Cytotoxic activities of Cyamopsis tetragonoloba L. seed extracts against huh-7 human liver cancer cells | ||||
| Assiut Journal of Agricultural Sciences | ||||
| Article 5, Volume 52, Issue 5, December 2021, Page 76-89 PDF (1.05 MB) | ||||
| Document Type: Research papers | ||||
| DOI: 10.21608/ajas.2022.106957.1066 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sally El-Shafei   ; Hosny S. Abd El-Salam; Gamal F. Abd El-Naem; Yasmine M. Ramadan
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| Department of Agricultural Chemistry, Faculty of Agriculture, Minia University | ||||
| Abstract | ||||
| Finding of new effective anti-cancer agents has become a top goal around the world. Thus, the objective of this study was to investigate the cytotoxic activities of guar seeds methanolic extract (GSME), guar seeds oil (GSO), guar gum (GG) and guar saponin (GSP) from seeds against huh-7 cancer cell line. For 72 hours, cells were exposed to 0.01, 0.1, 1.0, 10, 100 µg/ml of various guar seeds extracts (GSME, GSO, GG, and GSP), then the SRB assay was performed three times in triplicate to determine cell viability %, cell growth inhibition % and half-maximal inhibitory concentration (IC50). In addition, phase contrast inverted microscopy was used to examine the morphological changes in the cells. The huh-7 cells were shown to be cytotoxic to all guar seed extracts. Among the various examined preparations, guar seed saponin (GSP) was shown to be the most cytotoxic substance at a concentration of 100 µg/ml towards huh-7 cells. As a result, the guar seed tested extracts could potentially be used in the creation of anticancer drugs. | ||||
| Keywords | ||||
| Cytotoxicity; liver cancer cells; guar seeds | ||||
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