The Effect of Bone Marrow Derived Mesenchymal Stem Cells Versus their Exosomes on Imiquimod-Induced Psoriasis-Like Skin Inflammation in Female Albino Rats: A Histological Study | ||||
Egyptian Journal of Histology | ||||
Article 33, Volume 46, Issue 2, June 2023, Page 939-952 PDF (1.91 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejh.2022.125141.1650 | ||||
View on SCiNiTO | ||||
Authors | ||||
Ahmed Samir Mohamed Bakr 1; Mona Raafat 2; Gehad hammouda3; Salwa Mohamed Abdeltawab4 | ||||
1Department of Histology and Cell Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt | ||||
2Histology and cell biology. Faculty of Medicine, Ain Shams University | ||||
3Dep. of Histology and Cell biology Ain Shams Univ. | ||||
4Department of Histology, Faculty of Medicine, Ain Shams University, Cairo, Egypt | ||||
Abstract | ||||
Introduction: Psoriasis is a chronic skin inflammatory disease. Bone marrow mesenchymal stem cells (BM-MSCs) and their derived exosomes are known for their immunomodulatory properties. Aim of the Work: To investigate the effect of locally injected BM-MSCs and their derived exosomes in female albino rats subjected to imiquimod (IMQ)-induced psoriasis-like skin inflammation. Materials and Methods: Fifty albino rats (40 females and 10 males) were used. The female animals were randomly classified into four groups; group I (control group), group II (IMQ group) where the rats received topical IMQ once daily for 5 consecutive days, group III (IMQ + BM-MSCs) where the rats received a dose of 1 million BM-MSCs on the first day only in addition to topical IMQ, and group IV (IMQ + BM-MSCs-derived exosomes) where the rats received purified concentrate of exosomes derived from BM-MSCs in addition to topical IMQ. The 10 male albino rats served as the source of BM-MSCs and their derived exosomes. After 5 days, all animals were sacrificed, and skin specimens were processed for light microscopic studies: H&E and immunohistochemical staining for Proliferating Cell Nuclear Antigen (PCNA) and Y-chromosome identification using Real-time PCR. Morphometric measurements and statistical analysis were done for the mean epidermal thickness, the mean count of PCNA-positive keratinocytes in the epidermis, and the mean area of dermal PCNA-positive reaction. Results: The general observations and microscopic examination of sections obtained from group II rats revealed psoriasis-like skin inflammatory reactions including acanthosis, parakeratosis, and marked inflammatory infiltrate. There was a statistically significant increase in the epidermal thickness and PCNA-positive reactions in group II compared to other groups. Groups III and IV showed significant improvement, however, group III showed almost normal histological structure. Conclusion: BM-MSCs and purified exosomes concentrate were shown to significantly ameliorate psoriasis-like inflammatory changes in the skin of animal models. | ||||
Keywords | ||||
Exosomes; imiquimod; mesenchymal stem cells; psoriasisز | ||||
Statistics Article View: 504 PDF Download: 332 |
||||