Using Cutting Enzymes to Investigate Genetic Variation in Two Local Chicken Breeds, Fayumi and Dandarawi
El-sayed, M., Assi, H., El-Hamamsy, S. (2022). Using Cutting Enzymes to Investigate Genetic Variation in Two Local Chicken Breeds, Fayumi and Dandarawi. EKB Journal Management System, 13(2), 29-34. doi: 10.21608/jacb.2022.119989.1019
M. A. El-sayed; Heba A. E. M. Assi; S. M. A. El-Hamamsy. "Using Cutting Enzymes to Investigate Genetic Variation in Two Local Chicken Breeds, Fayumi and Dandarawi". EKB Journal Management System, 13, 2, 2022, 29-34. doi: 10.21608/jacb.2022.119989.1019
El-sayed, M., Assi, H., El-Hamamsy, S. (2022). 'Using Cutting Enzymes to Investigate Genetic Variation in Two Local Chicken Breeds, Fayumi and Dandarawi', EKB Journal Management System, 13(2), pp. 29-34. doi: 10.21608/jacb.2022.119989.1019
El-sayed, M., Assi, H., El-Hamamsy, S. Using Cutting Enzymes to Investigate Genetic Variation in Two Local Chicken Breeds, Fayumi and Dandarawi. EKB Journal Management System, 2022; 13(2): 29-34. doi: 10.21608/jacb.2022.119989.1019

Using Cutting Enzymes to Investigate Genetic Variation in Two Local Chicken Breeds, Fayumi and Dandarawi

Journal of Agricultural Chemistry and Biotechnology
Article 2, Volume 13, Issue 2, February 2022, Page 29-34  XML PDF (1.09 MB)
Document Type: Original Article
DOI: 10.21608/jacb.2022.119989.1019
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Authors
M. A. El-sayed email orcid 1; Heba A. E. M. Assi2; S. M. A. El-Hamamsy3
1Animal Genetic Resources Department, National Gene Bank, Agricultural Research Center, Giza, Egypt,
2Animal Production Research Institute, Agricultural Research Center, Giza, Egypt,
3Biochemistry Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
Abstract
The aim of this study was to compare the genetic variation in two local chicken breeds, Fayumi and Dandarawi. Two hundred individuals (100, Fayumi and 100, Dandarawi) were tested. Live body weights were collected and recorded for each bird separately within genotype from zero to 16 weeks of age. Two genes for the Growth Hormone GH and the Growth Hormone Receptor GHR were used in 12 birds at random for each of the two chicken breeds. After MspI and HindIII digestion, four allele's privet patterns were found for the GH and GHR genes, allele C, T and allele A, B, respectively. For GH, there were 17 and 7 for the Fayoumi and Dandarawi breeds, respectively. While in GHR resulted were 25 and 15 bands with the Fayoumi and Dandarawi breeds, respectively. The frequency of TT (GH) and AA (GHR) in Fayumi chicken was 0.28 and 0.55, respectively. Also, in Dandarawi chicken, the frequency of these alleles was 0.21 and 0.56, respectively. There was no homozygous genotype CT for GH detected in both studied breeds. The Polymorphic Information Content PIC values of 0.49, 0.65, and 0.49, 0.57 for Fayumi and Dandarawi chicken breeds, respectively, for two genes GH and GHR. Some possible genes for improving body weight in various indigenous chicken breeds had already been identified and exploited for selection and breeding reasons.
Keywords
Growth Hormone GH gene; Growth Hormone Receptor GHR gene; cutting enzyme; native chicken breeds; Fayumi and Dandarawi
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