Direct PCR-Based Detection of Y Chromosome Strs Loci from Human Hair Samples | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Article 3, Volume 10, Issue 2, December 2018, Page 27-35 PDF (688.86 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsc.2018.22664 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Hassab El- Nabi S. E.1; Elroby A. M.2; Geba K. M.1 | ||||
| 1Faculty of Science, Menoufia University, Shebin El-Kom, Menofia, Egypt, P. O. Box 32511 | ||||
| 2General Authority of Forensic Evidence, Ministry of Interior, Abbassia, Cairo, Egypt, P. O. Box 11517 | ||||
| Abstract | ||||
| Direct-PCR is not common for Y chromosome in forensic laboratories in Egypt. The current investigation was carried out to evaluate the priority of direct PCR technique in Y-STR test over routine extraction method.Results showed that direct PCR over hair samples obtained from five persons who do not use any Hair waxes recorded higher scale of peak heights than those subjected to a prior-PCR DNA extraction. In addition, much better results for direct PCR were obtained when one hair was subjected to direct PCR amplification than using three hairs. This technique may solve the problems of rapid and cost-reducing genotyping of forensic samples, as well as may improve the fluorescence detection of some genetic loci. | ||||
| Keywords | ||||
| Direct PCR; DNA profiles; hair waxes; Y-STRs | ||||
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