In vitro antibacterial activity of ethanolic crude extracts of Capsicum annum against Staphylococcus aureus and Escherichia coli isolated from pus and stool samples at Ruhengeri Referral Hospital, Rwanda | ||||
| Novel Research in Microbiology Journal | ||||
| Volume 2, Issue 6, November and December 2018, Page 156-166 PDF (338.48 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/NRMJ.2018.22708 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Solange Gahongayire1; Ibrahim Ntulume1; Kagimbura Ervine2; Bizimana Évariste2; Adamu A. Aliero   3
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| 1Department of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University, Uganda | ||||
| 2Faculty of Applied fundamental sciences, Department of Biomedical Laboratory Science, Ruhengeri Institute of Applied Sciences, Rwanda | ||||
| 3Department of Biological Sciences, Faculty of Sciences, Kebbi State University of Science and Technology, Aliero, Kebbi State, Nigeria | ||||
| Abstract | ||||
| This work aimed at determining the antibacterial activity of ethanolic crude extracts of leaves and fruits of Capsicum annum against Staphylococcus aureus and Escherichia coli; isolated from pus and stools samples of patients in Ruhengeri Referral hospital, Rwanda. Fruits and leaves of C. annum samples were collected from Sina Gerard enterprise in Rulindo District in July, 2012. Plant samples were shade dried for 10 days, and then standard method was used for extraction using 96% ethanol as a solvent. Antibacterial activities of ethanolic crude extracts of fruits and leaves of C. annum were determined against E. coli and S. aureus using standard disc diffusion method. Minimum inhibitory concentrations (MIC) and Minimum bactericidal concentrations (MBC) were also determined. Results showed that crude extracts of leaves and fruits of C. annum had antibacterial efficacy against S. aureus and E. coli. The mean diameter of inhibition zones and standard deviation of fruits crude extract against S. aureus and E. coli ranged from 8.17± 0.15 to 11.0 ± 0.89 mm, and 14.0 ± 0.25 to 17.9 ± 0.35 mm; respectively, while those of leaves crude extract ranged from 9.7 ± 0.26 to 14.7± 0.25mm, and 8.7± 0.35 to 11.1 ± 0.38 mm; respectively, at increasing extract concentration from 50 to 200 mg/ ml. MIC of fruits ethanolic crude extract against E. coli and S. aureus was 25 and 12.5mg /ml; respectively, while MBC was found to be 50 and 25mg/ ml, respectively. However, MIC of leaves ethanolic crude extract against E. coli and S. aureus were 50 and 12.5mg/ ml respectively, while MBC was found to be 100 and 50 mg/ ml, respectively. We concluded from this study that, ethanolic crude extract of both fruits and leaves of C. annum collected from Rwanda had antibacterial potential against tested bacteria, thus could be used as sources of new drugs. | ||||
| Keywords | ||||
| Capsicum annum; bacteria; pus; stool; Antibacterial activity; Ruhengeri Referral Hospital; Rwanda | ||||
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