Jasonia montana Extracts Stimulate Cell Differentiation in HCT-116 and Caco-2 Cell Lines

Aidy, Esraa A.; Fathy, Zeinab; Ahmed, Wafaa Abdullah; El-Gendy, Saad; Abou-Bakr, Amany; Omran, Mervat; Al-Shafie, Tamer A.

doi:10.21608/ejchem.2022.113827.5252

	Jasonia montana Extracts Stimulate Cell Differentiation in HCT-116 and Caco-2 Cell Lines
Egyptian Journal of Chemistry
Article 18, Volume 65, Issue 12, December 2022, Page 165-174 PDF (733.65 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.113827.5252
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Authors
Esraa A. Aidy ¹; Zeinab Fathy²; Wafaa Abdullah Ahmed³; Saad El-Gendy³; Amany Abou-Bakr⁴; Mervat Omran ⁵; Tamer A. Al-Shafie ⁶
¹Biochemistry Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Egypt
²Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt
³Cancer Biology Department, National Cancer Institute, Cairo University, Egypt
⁴Pathology Department, National Cancer Institute, Cairo University, Egypt
⁵Department of Cancer Biology, Pharmacology Unit, National Cancer Institute, Cairo University, Cairo 11796, Egypt
⁶Department of Biochemistry, Faculty of Dentistry, Pharos University in Alexandria
Abstract
Unlike differentiated cells, cancer stem cells (CSCs) can generate malignancy. Conventional chemotherapy results in CSCs spreading causing metastasis and relapse. Differentiation is an emerging approach providing a safer strategy to eradicate CSCs. Jasonia montana is a medicinal plant that occurs in Sinai Peninsula in Egypt. Here, we used two human colorectal cancer cell lines; HCT-116 and Caco-2 to investigate the ability of methanolic and petroleum ether extracts of Jasonia montana to differentiate colon CSCs. Tandem Mass–Spectrometry (MS/MS) revealed that quercitrin and 5-Hydroxycostic acid represent the major extracts ingredients. Cytotoxicity assay (SRB) illustrated that petroleum ether extract inhibited Caco-2 cells (IC50=10 μg/ml), whereas, methanolic extract inhibited HCT-116 cells (IC50=20 μg/ml). On treating HCT-116 and Caco-2 cells with methanolic and petroleum ether extract respectively in diluted doses to alleviate their cytotoxicity, qRT-PCR showed a significant increase in CK20 expression and a significant decrease in CD44 expression, a significant decrease in Sox-2 and NOTCH-1 expression and a significant increase in PTEN expression. Furthermore, qRT–PCR showed a significant decrease in miR-21-5P and miR-10b-5P expression and significant increase in miR-34a-5P and miR-145 expression. Our results suggest, for the first time, that Jasonia montana methanolic and petroleum ether extracts can induce colorectal CSCs differentiation.
Keywords
Jasonia montana extracts; colorectal cancer cells; differentiation; microRNAs


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