Molecular identification and pathogenicity of Ralstonia solanacearum isolates collected from north west of Pakistan
Junaid, M., Bibi, A., Ahmad, M., Ali, M., Noor, Y. (2019). Molecular identification and pathogenicity of Ralstonia solanacearum isolates collected from north west of Pakistan. EKB Journal Management System, 3(2), 314-325. doi: 10.21608/nrmj.2019.30611
Muhammad Junaid; Ayesha Bibi; Musharaf Ahmad; Muhammad Ali; Yousaf Noor. "Molecular identification and pathogenicity of Ralstonia solanacearum isolates collected from north west of Pakistan". EKB Journal Management System, 3, 2, 2019, 314-325. doi: 10.21608/nrmj.2019.30611
Junaid, M., Bibi, A., Ahmad, M., Ali, M., Noor, Y. (2019). 'Molecular identification and pathogenicity of Ralstonia solanacearum isolates collected from north west of Pakistan', EKB Journal Management System, 3(2), pp. 314-325. doi: 10.21608/nrmj.2019.30611
Junaid, M., Bibi, A., Ahmad, M., Ali, M., Noor, Y. Molecular identification and pathogenicity of Ralstonia solanacearum isolates collected from north west of Pakistan. EKB Journal Management System, 2019; 3(2): 314-325. doi: 10.21608/nrmj.2019.30611

Molecular identification and pathogenicity of Ralstonia solanacearum isolates collected from north west of Pakistan

Novel Research in Microbiology Journal
Volume 3, Issue 2, March and April 2019, Page 314-325  XML PDF (278.8 K)
Document Type: Original Article
DOI: 10.21608/nrmj.2019.30611
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Authors
Muhammad Junaid email 1; Ayesha Bibi2; Musharaf Ahmad3; Muhammad Ali4; Yousaf Noor5
1MFSC, Peshawar, Department of Agriculture Ext., Govt. of Khyber Pakhtunkhwah, Pakistan
2Department of Soil Microbiology, DSPN, ARI Peshawar, Govt. of Khyber Pakhtunkhwah, Pakistan
3Department of Plant Pathology, The University of Agriculture, Peshawar, Pakistan
4Department of Soil Microbiology, DSPN, ARI Peshawar, Govt. of Khyber Pakhtunkhwah, Pakistan
5Cereal Crop Research Institute, Pirsabak, Nowshera, Govt. of Khyber Pakhtunkhwah, Pakistan
Abstract
In the tomato commercial growing districts of Khyber Pakhtunkhwa (KP); a province in the north west of Pakistan, multiple comprehensive surveys were conducted during 2012. The main objectives of the current study were to identify the Ralstonia solanacearum (R. solanacearum) isolate through its colony characteristics, molecular tools; and to investigate the ability of this pathogen to cause Bacterial wilt (BW) disease, when being inoculated into tomato plant using different inoculation methods. For this purpose, a total of 74 locations covering all over the KP were visited for the presence of tomato plants with BW disease, caused by R. solanacearum. The bacterial pathogen was isolated from diseased plant tissues by growing it on the selective 2,3,5-triphenyl-tetrazolium chloride (TTC) medium. Based on colony morphology of R. solanacearum on the agar plates; and pathogenicity assays, about 29 isolates were guessed to be R. solanacearum. To further confirm the identity of these isolates, a species-specific primers-mediated Polymerase chain reaction (PCR) was carried out. Two specific primers i.e. forward primer: 5'GTCGCCGTCAACTCACTTTCC3', and reverse primer: 5'GTCGCCGTAGCAATGCGGAATCG3', were used for amplification of the 281bp band. Twenty five isolates out of the 29 were genetically confirmed to be R. solanacearum based on their amplified 281bp band. 
Keywords
Tomato; Bacterial wilt; Ralstonia solanacearum; Pathogenicity; PCR
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