Cryopreservation-induced changes in buffalo spermatozoa with special reference to their DNA, membrane, acrosomal and mitochondrial integrities
Anwer, A., Anwer, A., El-Badry, D., Zain, A. (2022). Cryopreservation-induced changes in buffalo spermatozoa with special reference to their DNA, membrane, acrosomal and mitochondrial integrities. EKB Journal Management System, 2(3), 1-8. doi: 10.21608/sjyr.2022.227617
Ahmed Anwer; Abeer Anwer; Diya El-Badry; Alaa Zain. "Cryopreservation-induced changes in buffalo spermatozoa with special reference to their DNA, membrane, acrosomal and mitochondrial integrities". EKB Journal Management System, 2, 3, 2022, 1-8. doi: 10.21608/sjyr.2022.227617
Anwer, A., Anwer, A., El-Badry, D., Zain, A. (2022). 'Cryopreservation-induced changes in buffalo spermatozoa with special reference to their DNA, membrane, acrosomal and mitochondrial integrities', EKB Journal Management System, 2(3), pp. 1-8. doi: 10.21608/sjyr.2022.227617
Anwer, A., Anwer, A., El-Badry, D., Zain, A. Cryopreservation-induced changes in buffalo spermatozoa with special reference to their DNA, membrane, acrosomal and mitochondrial integrities. EKB Journal Management System, 2022; 2(3): 1-8. doi: 10.21608/sjyr.2022.227617

Cryopreservation-induced changes in buffalo spermatozoa with special reference to their DNA, membrane, acrosomal and mitochondrial integrities

مجلة سوهاج لشباب الباحثين
Volume 2, Issue 3, 2022, Page 1-8  XML PDF (365.76 K)
Document Type: المقالة الأصلية
DOI: 10.21608/sjyr.2022.227617
View on SCiNiTO View on SCiNiTO
Authors
Ahmed Anwer email 1; Abeer Anwer2; Diya El-Badry3; Alaa Zain1
1Theriogenology Department, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt 825252
2Immunopharmacology Unit and Department of Artificial insemination and embryo transfer, Animal Reproduction Research Institute, Cairo, Egypt
3Department of Artificial insemination and embryo transfe, Animal Reproduction Research In-stitute, Cairo, Egypt
Abstract
The present study aimed to study the effect of cryopreservation on the membrane, acrosome, DNA, and mitochondrial integrities of buffalo bull spermatozoa. Two adult buffalo bulls were enrolled in this study. Semen samples were collected once weekly from each bull over a period of six successive weeks by means of artificial vagina.  Samples were processed for cryopreservation. Individual motility, mitochondrial activity as well as membrane, acrosomal and DNA integrities of fresh and frozen-thawed spermatozoa were evaluated. Results showed that the procedures applied for freeze-thawing resulted in significant (P ≤ 0.001) reduction in sperm motility, sperm membrane, acrosome, mitochondrial and DNA integrities by 26.67, 29.17, 17.66, 40 and 4.11%, respectively. In conclusion, the process of freezing and thawing significantly reduced semen quality of buffalo bull in terms of various functional parameters including motility, membrane, acrosome, DNA and mitochondrial integrities.
Keywords
Cryopreservation; Buffalo; Spermatozoa; DNA; Mitochondria
Statistics
Article View: 94
PDF Download: 113