Detection and identification of a new isolate of Grapevine fanleaf Virus naturally infecting Grapevine plants in Egypt using qReal Time-PCR
Gamil Aseel, D., Hamdy Abd El-Aziz, M., Hafez, E. (2019). Detection and identification of a new isolate of Grapevine fanleaf Virus naturally infecting Grapevine plants in Egypt using qReal Time-PCR. EKB Journal Management System, 3(6), 493-501. doi: 10.21608/nrmj.2019.66742
Dalia Gamil Aseel; Mahmoud Hamdy Abd El-Aziz; E. E. Hafez. "Detection and identification of a new isolate of Grapevine fanleaf Virus naturally infecting Grapevine plants in Egypt using qReal Time-PCR". EKB Journal Management System, 3, 6, 2019, 493-501. doi: 10.21608/nrmj.2019.66742
Gamil Aseel, D., Hamdy Abd El-Aziz, M., Hafez, E. (2019). 'Detection and identification of a new isolate of Grapevine fanleaf Virus naturally infecting Grapevine plants in Egypt using qReal Time-PCR', EKB Journal Management System, 3(6), pp. 493-501. doi: 10.21608/nrmj.2019.66742
Gamil Aseel, D., Hamdy Abd El-Aziz, M., Hafez, E. Detection and identification of a new isolate of Grapevine fanleaf Virus naturally infecting Grapevine plants in Egypt using qReal Time-PCR. EKB Journal Management System, 2019; 3(6): 493-501. doi: 10.21608/nrmj.2019.66742

Detection and identification of a new isolate of Grapevine fanleaf Virus naturally infecting Grapevine plants in Egypt using qReal Time-PCR

Novel Research in Microbiology Journal
Volume 3, Issue 6, November and December 2019, Page 493-501  XML PDF (411.06 K)
Document Type: Original Article
DOI: 10.21608/nrmj.2019.66742
View on SCiNiTO View on SCiNiTO
Authors
Dalia Gamil Aseel email 1; Mahmoud Hamdy Abd El-Azizorcid 2; E. E. Hafez1
1Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg EL-Arab city, Alexandria, Egypt
2Plant Pathology Institute, Agricultural Research Center, Alexandria, Egypt
Abstract
Grapevine fanleaf virus (GFLV) is a member of the genus Nepovirus in the family Comoviridae, a widely distributed virus responsible for grapevine (Vitis vinifera) degeneration. This virus causes serious economic losses by reducing grape crop yield. The Quantitative Real-Time Reverse Transcription-Polymerase Chain Reaction (qReal Time-PCR) assay was carried out on (GFLV) recovered from infected grapevines leaves at Alexandria, Egypt. A 606 bp fragment of the GFLV RNA-2 coat protein (CP) gene was amplified and then sequenced. Results of reactions of diagnostic hosts were observed on Gomphrena globosa, which developed systemic mottling, leaves twisting and necrotic spots during spring, whereas Chenopodium amaranticolor induced systemic mottling and leaf deformation, and its sap seemed relatively insensitive to the inhibitors of infection. Mottling of Glycine max was detected after inoculation, but inoculation of Nicotiana glutinosa didn't induce any symptoms. This study aimed to detect and identify a new isolate of GFLV-DA3 from Egypt using biological and molecular tools. 
Keywords
Grapevine fanleaf virus; qReal-Time PCR; Coat protein; Sequencing; Mechanical transmission
Statistics
Article View: 127
PDF Download: 76