Role of the JC Polyomavirus (JCV) and BK Polyomavirus (BKV) in the colorectal cancer of some Egyptian patients
Samir, S., B. Barakat, A., M. El-Senousy, W., A. Abou-Zeid, A., A. Rabiee, O. (2020). Role of the JC Polyomavirus (JCV) and BK Polyomavirus (BKV) in the colorectal cancer of some Egyptian patients. EKB Journal Management System, 4(1), 598-605. doi: 10.21608/nrmj.2020.73428
Sherihan Samir; Ahmed B. Barakat; Waled M. El-Senousy; Ahmed A. Abou-Zeid; Omar A. Rabiee. "Role of the JC Polyomavirus (JCV) and BK Polyomavirus (BKV) in the colorectal cancer of some Egyptian patients". EKB Journal Management System, 4, 1, 2020, 598-605. doi: 10.21608/nrmj.2020.73428
Samir, S., B. Barakat, A., M. El-Senousy, W., A. Abou-Zeid, A., A. Rabiee, O. (2020). 'Role of the JC Polyomavirus (JCV) and BK Polyomavirus (BKV) in the colorectal cancer of some Egyptian patients', EKB Journal Management System, 4(1), pp. 598-605. doi: 10.21608/nrmj.2020.73428
Samir, S., B. Barakat, A., M. El-Senousy, W., A. Abou-Zeid, A., A. Rabiee, O. Role of the JC Polyomavirus (JCV) and BK Polyomavirus (BKV) in the colorectal cancer of some Egyptian patients. EKB Journal Management System, 2020; 4(1): 598-605. doi: 10.21608/nrmj.2020.73428

Role of the JC Polyomavirus (JCV) and BK Polyomavirus (BKV) in the colorectal cancer of some Egyptian patients

Novel Research in Microbiology Journal
Volume 4, Issue 1, January and February 2020, Page 598-605  XML PDF (221.59 K)
Document Type: Original Article
DOI: 10.21608/nrmj.2020.73428
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Authors
Sherihan Samir email 1; Ahmed B. Barakat1; Waled M. El-Senousy2; Ahmed A. Abou-Zeid3; Omar A. Rabiee1
1Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
2Water Pollution Research Department, Environmental Research Division, National Research Centre (NRC), Cairo, Egypt
3Department of General Surgery, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Abstract
Although several cases and case-control studies reported the prevalence of JC polyomavirus (JCV) and BK polyomavirus (BKV) in tumor tissues of colorectal cancer (CRC) patients as well as in control samples; however, many recent studies did not show the prevalence of these viruses in the CRC patients. A total of 50 clinical biopsies samples were collected from patients with and without CRC in the General surgery department, Faculty of Medicine, Ain Shams University, Egypt, with the approval of the research ethics committee. Current results showed the absence of the genomic DNA of both JCV and BKV in the tumor tissue samples, adjacent-tumor normal mucosa samples from CRC patients, and also in the normal mucosa samples collected from the non-CRC patients. Moreover, results of the semi-nested PCR and real-time PCR are similar, and confirmed that JCV and BKV are not prevalent in the Egyptian CRC patients. There are no promising results for further investigation of the expression of the large T-Ag protein, and any other related proteins in the tumor cells. Based on the present results, no evidence supports the association between the JCV, BKV and CRC in the Egyptian patients. For the best of our knowledge, this is the first case-control study on the prevalence of JCV and BKV in the Egyptian CRC patients. The aim of the current study was to detect the presence of JCV and BKV genomic DNA in the tissue samples, to support further studies on the role of these viruses in the CRC etiology and development.
Keywords
Polyomaviruses; Colorectal cancer; Duplex semi-nested PCR; Egyptian patients
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