Colistin susceptibility among multidrug resistant Gram-negative bacilli isolated from Tertiary hospital in Egypt | ||||
| Novel Research in Microbiology Journal | ||||
| Volume 4, Issue 5, September and October 2020, Page 968-978 PDF (378.79 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/nrmj.2020.118447 | ||||
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| Authors | ||||
Shimaa A. Abdel Salam1; Raghda Hager   2
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| 1Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University- Egypt | ||||
| 2Department of Medical Microbiology and Immunology, Faculty of Medicine, Misr University for Science and Technology, Egypt | ||||
| Abstract | ||||
| Colistin is considered as the last resort for treatment of bacterial infections caused by carbapenem-resistant Gram-negative bacilli (GNB). Colistin resistance can increase due to the spread of plasmid-borne mcr-1 gene. This study aimed to determine colistin susceptibility and to detect the presence of mcr-1 gene in the clinical isolates of GNB recovered from different clinical samples collected from Ain Shams University Hospital, Cairo, Egypt. About thirty-five GNB isolates were recovered from the different clinical samples that were collected during the period from February-April, 2019. These isolates were subjected to antibiotic susceptibility testing using disc diffusion assay, and colistin susceptibility through the E-test. In addition, conventional Polymerase chain reaction (PCR) was carried out for detection of mcr-1 gene among the colistin GNB resistant isolates. Most of the GNB isolates (60 %) were recovered from blood samples. Klebsiella pneumoniae (K. pneumoniae) was the most common isolated bacterium; that was represented by 24 isolates (68%). Out of the 35 GNB, only five isolates (14.3 %) were resistant to colistin by E-test, with MIC >256 𝜇g/ ml. The mcr-1 gene was detected only in one Pseudomonas aeruginosa (P. aeruginosa) isolate. This study concluded the low frequency of mcr-1 gene among the current GNB isolates. However, a large scale study is recommended to detect colistin resistance among GNB. | ||||
| Keywords | ||||
| Colistin susceptibility; mcr-1 gene; Multi-drug resistant GNB; PCR | ||||
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