Glucophilic fungi of sugarcane bagasse in Egypt | ||||
Journal of Environmental Studies | ||||
Article 3, Volume 25, Issue 1, June 2021, Page 39-50 PDF (674.57 K) | ||||
Document Type: High quality original papers | ||||
DOI: 10.21608/jesj.2021.228288 | ||||
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Authors | ||||
Mohamed S. Youssef ![]() | ||||
1Botany and Microbiology Department, Faculty of Science, Sohag University 82524, Sohag, Egypt. | ||||
2Microbiology Department, Soil, Water & Environment Research Institute, Agricultural Research Center, Giza, Egypt. | ||||
3Microbiology Department, Soil, Water & Environment Research Institute, Agricultural Research Center, Shandaweel Research Station, Sohag, Egypt. | ||||
Abstract | ||||
Thirty sugarcane bagasse samples were collected from different sugarcane juice mills in Sohag Governorate, Upper Egypt. The moisture content of tested samples was fluctuated between 32.58 - 70.59%. Total carbohydrates were estimated, and the richest sample was No. 21 with 535.10 mg/g dry weight, whereas the lowest sample was No. 3 with 508.131 mg/g dry weight. Soluble sugars of different samples were determined using spectrophotometer at 620 nm. The soluble sugars content ranged between 120 - 160 mg/g dry weight. Dilution- (8 genera, 27 species and 4 varieties) and direct- (8 genera, 25 species and 4 varieties) plate methods on glucose-Czapek's agar medium at 28 ±2 oC were used for isolation of bagasse glucophilic fungi. It was found that the total fungal populations in case of dilution–plate method (69720 cfu/g) was so high than those of direct–plate method (828 cfu/ 20 bagasse segments). Aspergillus was appeared in high population and was parallel to those of total fungal counts (51700 cfu/g, 74.15 % of total count, in 29 samples with 96.7% of the samples in dilution method and 731 cfu/ 20 segments, 88.3% of total count, in 30 samples with 100% of the tested samples using direct-plate method. | ||||
Keywords | ||||
sugarcane bagasse; fungi; total and soluble carbohydrates | ||||
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