Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital
sultan, L., Eshra, K., Mahgoub, F., Eshra, K. (2022). Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital. EKB Journal Management System, 31(2), 7-11. doi: 10.21608/ejmm.2022.228613
laila taher sultan; kareman A Eshra; Fawkia Mohamed Mahgoub; kareman Ahmed Eshra. "Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital". EKB Journal Management System, 31, 2, 2022, 7-11. doi: 10.21608/ejmm.2022.228613
sultan, L., Eshra, K., Mahgoub, F., Eshra, K. (2022). 'Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital', EKB Journal Management System, 31(2), pp. 7-11. doi: 10.21608/ejmm.2022.228613
sultan, L., Eshra, K., Mahgoub, F., Eshra, K. Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital. EKB Journal Management System, 2022; 31(2): 7-11. doi: 10.21608/ejmm.2022.228613

Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital

Egyptian Journal of Medical Microbiology
Article 2, Volume 31, Issue 2, April 2022, Page 7-11  XML PDF (360.29 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.228613
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Authors
laila taher sultan1; kareman A Eshra2; Fawkia Mohamed Mahgoub1; kareman Ahmed Eshra email 1
1medical microbiology and immunology , faculty of medicine, Tanta university
2Department of Surgery and burn Faculty of Medicine, Tanta University, Egypt
Abstract
Background: Multi-Drug Resistant (MDR) P. aeruginosa is a major health problem in burn infection. Exotoxin A gene plays an important role in pathogenesis of burn infections Objective: the isolation of MDR p.aeruginosa from infected burns and Detection of Exotoxin-A (Exo-A) gene in MDR P. aeruginosa isolates using conventional Polymerase Chain Reaction (PCR). Methods: The study was carried out on 200 samples of burn patients in a form of burn swabs, these patients admitted to Burn Units at Tanta university Hospitals. PCR was done on isolated MDR P.aeruginosa for detection of exotoxin- A gene. Results: P. aeruginosa was the most common organism isolated from burn infection with a percentage of 32.5% .96.3% of them contained the EXO-A gene while only two cases (3.3%) didn't contain the gene. Conclusion: EXO-A gene is an important virulence factor of P.aeruginosa infected burns. MDR P. aeruginosa incidence increased in hospitals.
Keywords
Burn wound; P.aeruginosa; MDR; EXO-A gene
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