A one year single-center experience on Stenotrophomonas maltophilia strains in Alexandria, Egypt
Meheissen, M., Morsi, M., Hamad, M., Raouf, M. (2022). A one year single-center experience on Stenotrophomonas maltophilia strains in Alexandria, Egypt. EKB Journal Management System, 31(2), 69-76. doi: 10.21608/ejmm.2022.228827
Marwa Ahmed Meheissen; Mona Morsi; Mabrouka Hamad; May Raouf. "A one year single-center experience on Stenotrophomonas maltophilia strains in Alexandria, Egypt". EKB Journal Management System, 31, 2, 2022, 69-76. doi: 10.21608/ejmm.2022.228827
Meheissen, M., Morsi, M., Hamad, M., Raouf, M. (2022). 'A one year single-center experience on Stenotrophomonas maltophilia strains in Alexandria, Egypt', EKB Journal Management System, 31(2), pp. 69-76. doi: 10.21608/ejmm.2022.228827
Meheissen, M., Morsi, M., Hamad, M., Raouf, M. A one year single-center experience on Stenotrophomonas maltophilia strains in Alexandria, Egypt. EKB Journal Management System, 2022; 31(2): 69-76. doi: 10.21608/ejmm.2022.228827

A one year single-center experience on Stenotrophomonas maltophilia strains in Alexandria, Egypt

Egyptian Journal of Medical Microbiology
Article 12, Volume 31, Issue 2, April 2022, Page 69-76  XML PDF (248.08 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.228827
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Authors
Marwa Ahmed Meheissen email orcid 1; Mona Morsi2; Mabrouka Hamad3; May Raouf4
1Medical Microbiology & Immunology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
2Medical Microbiology and Immunology Department, Faculty of Medicine, University of Alexandria, Egypt
3Medical Microbiology and Immunology, Microbiology Department, Faculty of Medicine, Sirte University, Sirte, Libya.
4Medical Microbiology and Immunology Department, Faculty of Medicine, University of Alexandria, Egypt.
Abstract
Background & objectives: Stenotrophomonas maltophilia (S. maltophilia) emerged as an important opportunistic nosocomial multidrug resistant pathogen. This study aimed to investigate S. maltophilia strains to determine their virulence factors, antimicrobial resistance pattern, detect integrons and reveal their genotypic relatedness. Methodology: S. maltophilia clinical isolates were subjected to antimicrobial susceptibility testing using the VITEK-2 compact system (BioMérieux). Biofilm production, hemolytic, protease and lipase activity were tested.  Polymerase Chain Reaction (PCR) for integrase 1 and 2 genes and Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR were done. Results:S. maltophilia constituted 0.8% of gram-negative non-fermenters. All strains were biofilm producers. All strains were susceptible to trimethoprim/sulfamethoxazole. Class 1 integron was detected in five (35.7%) strains. ERIC PCR showed high genetic diversity between the strains. Conclusions: Although multiple virulence factors were detected, strains were still susceptible to the recommended antimicrobials. ERIC PCR was found to be valuable in detecting genetic diversity among S. maltophilia strains being easy, rapid, cheap and available technique. 
Keywords
Stenotrophomonas maltophilia; MALDITOF; virulence factors; ERIC PCR; epidemiology
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