A potential prophylactic strategy of anti-schistosomal immunization using nanotechnology in murine models
Etewa, S., Al-Hoot, A., Sharaf, H., Mohammad, S., Moawad, H., Sarhan, M., El-Shafey, M., Senosy, H. (2018). A potential prophylactic strategy of anti-schistosomal immunization using nanotechnology in murine models. EKB Journal Management System, 11(3), 162-174. doi: 10.21608/puj.2018.6072.1023
Samia Etewa; Abd-Allah Al-Hoot; Hesham Sharaf; Samira Mohammad; Howayda Moawad; Mohamed Sarhan; Mahmoud El-Shafey; Huda Senosy. "A potential prophylactic strategy of anti-schistosomal immunization using nanotechnology in murine models". EKB Journal Management System, 11, 3, 2018, 162-174. doi: 10.21608/puj.2018.6072.1023
Etewa, S., Al-Hoot, A., Sharaf, H., Mohammad, S., Moawad, H., Sarhan, M., El-Shafey, M., Senosy, H. (2018). 'A potential prophylactic strategy of anti-schistosomal immunization using nanotechnology in murine models', EKB Journal Management System, 11(3), pp. 162-174. doi: 10.21608/puj.2018.6072.1023
Etewa, S., Al-Hoot, A., Sharaf, H., Mohammad, S., Moawad, H., Sarhan, M., El-Shafey, M., Senosy, H. A potential prophylactic strategy of anti-schistosomal immunization using nanotechnology in murine models. EKB Journal Management System, 2018; 11(3): 162-174. doi: 10.21608/puj.2018.6072.1023

A potential prophylactic strategy of anti-schistosomal immunization using nanotechnology in murine models

Parasitologists United Journal
Article 7, Volume 11, Issue 3, December 2018, Page 162-174  XML PDF (699.76 K)
Document Type: Original Article
DOI: 10.21608/puj.2018.6072.1023
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Authors
Samia Etewa1; Abd-Allah Al-Hoot2; Hesham Sharaf3; Samira Mohammad4; Howayda Moawadorcid 5; Mohamed Sarhan email orcid 6; Mahmoud El-Shafey7; Huda Senosy8
1Departments of Medical arasitology,Faculties of Medicine and Science, Zagazig University, Zagazig 44519, Egypt
2Departments of Medical Zoology Faculties of Medicine and Science, Zagazig University, Zagazig 44519, Egypt
3Departments of Medical Zoology Faculties of Medicine,and Science, Zagazig University, Zagazig 44519, Egypt
4Departments of Medical Parasitology ,Faculties of Medicine and Science, Zagazig University, Zagazig 44519, Egypt
5Departments of Medical Parasitology,, Faculties of Medicine and Science, Zagazig University, Zagazig 44519, Egypt
6Departments of Medical Parasitology , Faculties of Medicine and Science, Zagazig University, Zagazig 44519, Egypt
7Departments of Clinical Pathology,Faculties of Medicine and Science, Zagazig University, Zagazig 44519, Egypt
8Departments of ,Zoology,Faculties of Medicine and Science, Zagazig University, Zagazig 44519, Egypt
Abstract
Background:The emergence of chemotherapy resistant schistosomes necessitates finding new prophylaxis strategies based on immunization by using a modified vaccine candidate.
Objective: To assess thepotential of an anti-schistosomal vaccine modified by nanotechnology.
Materials and Methods: Soluble adult worm antigen preparation (SWAP) either crude or/and loaded on chitosan nanoparticles (CSNPs)were used. The anti-schistosomal impact was assessed in terms of stools egg count,worm burden and ultrastructural studyof juvenile and adult schistosomes.
Results:Chitosan nanoparticles potentiated SWAP efficacy to exceed the impact of crude SWAP.The combination of SWAP loaded on CSNPs and crude SWAP showed the best immunization effects regarding adult worm number andfecundity,as well as morphological and tegumental distortion of juvenile than adult stages.
Conclusion:Modified SWAP on CSNPs gave better results in anti-schistosome immunization thancrude SWAP.Immunization by combining different antigens gave the best results.
Keywords
Chitosan nanoparticles; Immunization; schistosomes; SWAP; ultrastructural study
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