Studies On Bean Yellow Mosaic Virus Infecting Some Leguminous Crops In Egypt
El-Helaly, S., Awad, H., Ahmed, A., Al-Attar, A. (2016). Studies On Bean Yellow Mosaic Virus Infecting Some Leguminous Crops In Egypt. EKB Journal Management System, 11(2), 1-9. doi: 10.21608/ejcp.2016.229440
Sahar El-Helaly; Hosam Awad; Amal Ahmed; ahmed Al-Attar. "Studies On Bean Yellow Mosaic Virus Infecting Some Leguminous Crops In Egypt". EKB Journal Management System, 11, 2, 2016, 1-9. doi: 10.21608/ejcp.2016.229440
El-Helaly, S., Awad, H., Ahmed, A., Al-Attar, A. (2016). 'Studies On Bean Yellow Mosaic Virus Infecting Some Leguminous Crops In Egypt', EKB Journal Management System, 11(2), pp. 1-9. doi: 10.21608/ejcp.2016.229440
El-Helaly, S., Awad, H., Ahmed, A., Al-Attar, A. Studies On Bean Yellow Mosaic Virus Infecting Some Leguminous Crops In Egypt. EKB Journal Management System, 2016; 11(2): 1-9. doi: 10.21608/ejcp.2016.229440

Studies On Bean Yellow Mosaic Virus Infecting Some Leguminous Crops In Egypt

Egyptian Journal of Crop Protection
Volume 11, Issue 2, December 2016, Page 1-9  XML PDF (541.31 K)
Document Type: Research articles
DOI: 10.21608/ejcp.2016.229440
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Authors
Sahar El-Helaly email 1; Hosam Awad1; Amal Ahmed2; ahmed Al-Attar2
1Department of Agricultural Botany ,Faculty of Agriculture,Menoufia University,Egypt
2Department of Virus and Phytoplasma Research, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
Abstract
Bean yellow mosaic virus (BYMV) was isolated from naturally infected bean
(phaseolus valgaris, L. cv. Karnk) and faba bean (Vicia faba, L cv. Balady) plants
were collected from different districts in menoufia governorate, Egypt; showing vein
chlorosis followed by green and/or yellow mosaic symptoms that may be due to virus
infection. Diseased samples were subjected to identification of isolated virus based
on symptomatology host range, serological tests (DAS- ELISA, Tissue blot
immunoassay TBIA and electron microscopy). Histological studies of bean infected
leaves by BYMV cleared that chloroplasts contained starch grain turned to spherical
in shape and with lost of their envelopes totally or partially. Chlorophyll a, b and
carotenoids as well as oxidative enzymes (peroxidase and polyphenol oxidase) were
determined in viral infected leaves. Molecular detection of BYMV using specific
primers was detected using RT-PCR; its products showed a single amplified
fragment of ~ 900 bp in electrophoresis analysis.
Keywords
Bean yellow mosaic virus (BYMV); faba bean; bean; symptoms; serological tests; DAS-ELISA; TBIA electron microscopy; chlorophyll; enzymes and RT-PCR
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