Epidemiology of hepatitis B virus infection among Pregnant Women in Lubumbashi, Democratic Republic of Congo: Prevalence, risk factors, and Genotype Distribution | ||||
| African Journal of Gastroenterology and Hepatology | ||||
| Volume 5, Issue 1, January 2022, Page 19-32 PDF (557.35 K) | ||||
| Document Type: Original Clinical | ||||
| DOI: 10.21608/ajgh.2022.120953.1002 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
ARSENE AKT KABAMBA   1; Christian CK Kakisingi2; Claude CM Mwamba2; Christophe CN Nyembo2; François FD Dufrasne3; Géraldine GD Dessilly3; Benoit BK KABAMBA3; Albert AL Longanga1
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| 1Faculty of Pharmaceuticals Sciences, University of Lubumbashi | ||||
| 2Faculty of Medecine, University of Lubumbashi | ||||
| 3Catholic University of Louvain, Microbiology Laboratory | ||||
| Abstract | ||||
| Mother-to-child transmission of hepatitis B virus is a major worldwide public health concern. This study aimed to determine the seroprevalence and identify the risk factors of carrying hepatitis B surface Antigen in pregnant women. Furthermore to determine the biochemical modifications and study the distribution of hepatitis B Virus genotypes among pregnant women living with hepatitis B Virus in Lubumbashi. Detection of HBsAg was carried out by rapid diagnostic test. Biochemical analysis was performed using spectrophotometric method. The qPCR was performed on the LightCycler® 96 analyzer. The sequencing reaction on amplicons from the overlap region of the P and S genes was carried out on the Veriti™ Thermal Cycler. The hepatitis B Virus Desoxyribonucleic Acid sequencing was achieved on an ABI PRISM 3500 analyzer. The seroprevalence of HBV among pregnant women was 4.4%. Blood transfusion and unprotected sex have been significantly associated with the risk of carrying HBsAg. Increased level of bilirubin and transaminases were observed. The genotypes E (59.4%), A (40.6%) and some drug resistance mutations were identified in the study population. With an HBV seroprevalence of 4.4%, Mother to child transmission of HBV remains a public health concern in Lubumbashi. Therefore, efforts aiming at reducing HBV infection among pregnant women and children should be strongly encouraged. Data obtained from hepatitis B Virus genotyping and the resistance mutations should help health authorities as a tool towards the adoption of treatment options including vaccination strategies and the development of a mapping of HBV genotypes in the Democratic Republic of Congo. | ||||
| Keywords | ||||
| Epidemiology; Genotypes; Hepatitis B Virus; Pregnant women | ||||
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