Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate
Abd Elkarim, M., Hegazy, E., Eshra, K., Zamzam, A. (2022). Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate. EKB Journal Management System, 3(4), 1032-1043. doi: 10.21608/mid.2022.126007.1255
Mai Abd Elkarim; Eman E. Hegazy; kareman Ahmed Eshra; Afaf Sayed Ahmed Zamzam. "Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate". EKB Journal Management System, 3, 4, 2022, 1032-1043. doi: 10.21608/mid.2022.126007.1255
Abd Elkarim, M., Hegazy, E., Eshra, K., Zamzam, A. (2022). 'Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate', EKB Journal Management System, 3(4), pp. 1032-1043. doi: 10.21608/mid.2022.126007.1255
Abd Elkarim, M., Hegazy, E., Eshra, K., Zamzam, A. Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate. EKB Journal Management System, 2022; 3(4): 1032-1043. doi: 10.21608/mid.2022.126007.1255

Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate

Microbes and Infectious Diseases
Article 30, Volume 3, Issue 4, November 2022, Page 1032-1043  XML PDF (404.92 K)
Document Type: Original Article
DOI: 10.21608/mid.2022.126007.1255
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Authors
Mai Abd Elkarim1; Eman E. Hegazy2; kareman Ahmed Eshra email orcid 2; Afaf Sayed Ahmed Zamzam2
1Forensic lab doctor in DNA unit in Tanta Department ,the Egyptian Forensic Medicine Authority
2Medical Microbiology and Immunology Department, Tanta University, Faculty of Medicine, Egypt.
Abstract
Background:Food borne illness represents a major public health problem associated with high morbidity and mortality rates. Methods: One hundred samples (foods and drinks) were collected in sterile stomacher bags, Bacterial isolation, colony count, antimicrobial susceptibility testing, and detection of Staphylococcus aureus (S.aureus) enterotoxin genes by multiplex PCR were done. Results: The current study revealed that the Gram-negative bacilli were the most frequent isolated organisms representing (63.3%) followed by Gram positive bacteria (34.5%) and fungi were the least found (2.2%). Among Gram negative bacilli, Klebsiella was the most frequently isolated bacteria, among Gram positive bacteria, Staphylococci were the most frequently isolated bacteria, all isolated fungi were Candida species. Out of 16 identified S. aureus strains recovered from the examined samples, 6 isolates were proved to be enterotoxigenic. One isolate carried Staphylococal enterotoxin C (SEC) gene, two isolates carried both Staphylococcal enterotoxin A (SEA) genes and Staphylococcal enterotoxin B (SEB) gene, and three isolates carried SEA, SEB, and SEC genes. None of the isolates contained SED gene. Conclusion: Based on the findings of this study, food borne illness represent a major public health problem in Tanta.
Keywords
Keywords: contamination of food and drink; Foodborne ilness; enterotoxins; multiplex PCR
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