Next-generation sequencing (NGS) detection of PIK3CA somatic mutations in Hepatocellular carcinoma (HCC) Egyptians | ||||
African Journal of Biological Sciences | ||||
Article 3, Volume 18, Issue 1, June 2022, Page 29-38 PDF (872.01 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ajbs.2022.230601 | ||||
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Authors | ||||
Amr M. Shugaa Addin 1; Randa M Talaat2; Moustafa A Sakr3; Mohamed K Khalifa2; Ehab A Ahmed4; Ghada M Nasr3; Manal O El Hamshary3 | ||||
1Molecular diagnostics and therapeutics department,GEBRI.University of Sadat city | ||||
2Children Cancer Hospital 57357, Egypt | ||||
3Department of Molecular Diagnostic and Therapeutic, Genetic Engineering & Biotechnology Research Institute, University of Sadat City, Egypt | ||||
4Chemistry department, Faculty of science, Cairo University, Egypt. | ||||
Abstract | ||||
Hepatocellular carcinoma (HCC) is the tertiary greatest communal malignant cancer that origins mortality globally. A high prevalence of return means that even while cancer is getting more treatable in its early stages, advanced cases have a bad prognosis. To properly treat HCC, it is now necessary to understand its pathogenic process and its associated genetic abnormalities. The next-generation sequencing (NGS) was used to discover PIK3CA somatic mutations in HCC Egyptian patients. In the present study a unique NGS panel (AmpliSeq) containing the PIK3CA gene was utilized to examine 21 liquid biopsy samples from patients with HCC. The results indicated that over 40 -single-nucleotide-variation (SNV) were recognized in PIK3CA gene with incidence of 85.7%. The changes were dispersed between deleterious, undefined significance, and tolerated deviations, where the preponderance of the changes were the missense variants (68%),synonymous(25%), and (7%) for stop-gained. It was concluded from this research that detection of numerous somatic mutations of PIK3CA can assist in the etiology of HCC. | ||||
Keywords | ||||
PIK3CA; HCC; Non-Synonymous; Mutation; SNV; NGS | ||||
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