Immunohistochemical markers study from different organs of rats after death for estimation of postmortem interval: An experimental study. | ||||
| Minia Journal of Medical Research | ||||
| Volume 32, Issue 2, April 2021, Page 80-90 PDF (1.1 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjmr.2021.231550 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Morid M. Hanna1; Soha A. Abd El Wahab2; Rana Adel1; Abeer M. Ahmed1 | ||||
| 1Department of Forensic Medicine & Toxicology; Faculty of Medicine - Minia University | ||||
| 2Department of Histology & Cell Biology; Faculty of Medicine - Minia University | ||||
| Abstract | ||||
| The present study aimed to estimate postmortem interval through immunohistochemical markers and histological examination of the skeletal muscle of rats at different time intervals. Fifty adult male albino rats were divided into five groups with 10 rats in each group. The rats were killed by cervical dislocation and the skeletal muscle samples were taken at times (zero, 24, 48, 72, and 96 hrs PM). The samples were subjected to histological and immunohistochemical examination. The results revealed that histological changes occurred gradually over the time since death. Immunohistochemical results of p53 and caspase-3 showed a significantly strong negative correlation with PMI. It was concluded that postmortem histological changes and immunohistochemistry of apoptotic markers (p53 and caspase-3) in skeletal muscle could be useful for estimating a postmortem interval. | ||||
| Keywords | ||||
| Postmortem interval; Forensic science; Caspase-3; P53; Apoptosis; Immunohistochemistry | ||||
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