Prevalence of multidrug resistant Escherichia coli recovered from patients suffering from urinary tract infections
Mahmoud Masoud, S., Mahmoud Abd El-Baky, R., A. Aly, S., Ali Ibrahem, R. (2022). Prevalence of multidrug resistant Escherichia coli recovered from patients suffering from urinary tract infections. EKB Journal Management System, 6(2), 1543-1556. doi: 10.21608/nrmj.2022.231801
Salwa Mahmoud Masoud; Rehab Mahmoud Abd El-Baky; Sherine A. Aly; Reham Ali Ibrahem. "Prevalence of multidrug resistant Escherichia coli recovered from patients suffering from urinary tract infections". EKB Journal Management System, 6, 2, 2022, 1543-1556. doi: 10.21608/nrmj.2022.231801
Mahmoud Masoud, S., Mahmoud Abd El-Baky, R., A. Aly, S., Ali Ibrahem, R. (2022). 'Prevalence of multidrug resistant Escherichia coli recovered from patients suffering from urinary tract infections', EKB Journal Management System, 6(2), pp. 1543-1556. doi: 10.21608/nrmj.2022.231801
Mahmoud Masoud, S., Mahmoud Abd El-Baky, R., A. Aly, S., Ali Ibrahem, R. Prevalence of multidrug resistant Escherichia coli recovered from patients suffering from urinary tract infections. EKB Journal Management System, 2022; 6(2): 1543-1556. doi: 10.21608/nrmj.2022.231801

Prevalence of multidrug resistant Escherichia coli recovered from patients suffering from urinary tract infections

Novel Research in Microbiology Journal
Volume 6, Issue 2, March and April 2022, Page 1543-1556  XML PDF (458.93 K)
Document Type: Original Article
DOI: 10.21608/nrmj.2022.231801
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Authors
Salwa Mahmoud Masoud1; Rehab Mahmoud Abd El-Bakyorcid 2; Sherine A. Aly3; Reham Ali Ibrahem email orcid 1
1Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt
2Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 11566, Egypt
3Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71516, Egypt
Abstract
Urinary tract infections are common human bacterial infections caused by Escherichia coli, which is one of the most frequent uropathogens. Treatment of such infections has become challenging due to the elevated levels of antimicrobial resistance. The aims of this work were to study the resistance pattern of E. coli pathogens recovered from patients with urinary tract infections attending to Minia University Hospital, Minia Governorate, Egypt, and to detect the co-existence of different extended spectrum β-lactamases (ESBLs) and metallo β-lactamases (MBLs) genes. The in vitro antibiotic sensitivity was tested using the disc diffusion assay. The production of ESBLs and MBLs was investigated through the combined disk and EDTA-combined disk synergy assays, while the molecular studies were carried out using the conventional polymerase chain reaction (PCR). High prevalence of ESBLs producers (61.6 %) was detected, whereas ESBLs and MBLs production was significantly associated with high multiple antimicrobial resistance index (MARI). The bla-TEM was the most prevalent genotype (94.6 %), while the prevalence of bla-NDM and bla-IMP was 45.9 % and 40.5 %, respectively. Imipenem and amikacin were the most effective antibacterial antibiotics. The current results showed statistically significant association of the tested genes, as 89 % of the E. coli isolates harbored more than one gene. This study highlighted a public health concern; as the elevated antibiotic resistance narrowed the therapeutic options and led to the failure of treatment of such bacterial infections. 
Keywords
Urinary tract infections; Uropathogenic E. coli; ESBLs; MBLs; MDR; XDR
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