Isolation of Helicobacter Pylori from Raw Milk and Study on Its Survival in Fermented Milk Products
Talaat Al Sherief, L., Thabet, S. (2022). Isolation of Helicobacter Pylori from Raw Milk and Study on Its Survival in Fermented Milk Products. EKB Journal Management System, 7(2), 73-81. doi: 10.21608/javs.2022.124671.1133
Lamiaa M. Talaat Al Sherief; Salwa S. Thabet. "Isolation of Helicobacter Pylori from Raw Milk and Study on Its Survival in Fermented Milk Products". EKB Journal Management System, 7, 2, 2022, 73-81. doi: 10.21608/javs.2022.124671.1133
Talaat Al Sherief, L., Thabet, S. (2022). 'Isolation of Helicobacter Pylori from Raw Milk and Study on Its Survival in Fermented Milk Products', EKB Journal Management System, 7(2), pp. 73-81. doi: 10.21608/javs.2022.124671.1133
Talaat Al Sherief, L., Thabet, S. Isolation of Helicobacter Pylori from Raw Milk and Study on Its Survival in Fermented Milk Products. EKB Journal Management System, 2022; 7(2): 73-81. doi: 10.21608/javs.2022.124671.1133

Isolation of Helicobacter Pylori from Raw Milk and Study on Its Survival in Fermented Milk Products

Journal of Applied Veterinary Sciences
Article 12, Volume 7, Issue 2, April 2022, Page 73-81  XML PDF (1013.38 K)
Document Type: Original Article
DOI: 10.21608/javs.2022.124671.1133
View on SCiNiTO View on SCiNiTO
Authors
Lamiaa M. Talaat Al Sherief; Salwa S. Thabet email
Food Hygiene Department, Animal Health Research Institute, Agriculture Research Center (ARC), Egypt.
Abstract
This research aimed to know if raw milk is a possible source of Helicobacter. pylori infection to human and assessing the inhibitory effect of fermented milk products on H. pylori growth. Sixty samples of cows' milk and raw marketable milk (30 samples each) were tested to detect H. pylori and were collected from farms and supermarkets in Assiut City, Egypt. The pathogen could be isolated from 13.33 and 6.66 % of cows' milk and marketable milk, respectively, by using the conventional culture method. Confirmation of the isolated strains using the PCR technique revealed that, 50% of the isolated strains were positive for the 16S rRNA gene. The strong antibiogram of Lactobacillus acidophilus was evident, where the count of the testedtwo strains of H. pylori (S1 and S2) was sharply decreased to 2.6 and 2.17 log cfu/ g yoghurt and 2.47 and 3.04 log cfu/ ml rayeb at the time of fermentation and could not be isolated at the first day. In case of Bifidobacterium bifidum, the count of S1 and S2 on the first day was 2.3 and 2.77 log cfu/ g yoghurt and 2.95 and 2.7 log cfu/ ml rayeb. Regarding the viability, H. pylori strains have remained viable for two days in yoghurt that was not supplemented with probiotics and could survive for seven days in control milk samples. There was no significant difference between the growth patterns of S1 and S2 in all treatments. Finally, fermented milk products containing probiotics were more effective in the survival of H. pylori than fermented milk products with no probiotics with a significant difference (P-value < 0.05).
Keywords
Helicobacter pylori; Milk; Probiotics; Rayeb; Yoghurt
References
ABDEL-LATIF, EMAN, F., MAROUF, S. A., and HAMZA, DALIA, A., 2016. Molecular and serological detection of H. pylori in cow’s milk and its impact on human health. I. J. B. P. A. S., 5: 60- 66.

ABO- DONIA, S. A. 2008. Origin, history and manufacture process of Egyptian dairy products: An overview. Alexandria J. Food Sci. Technol., 5: 51- 62.

AZEVEDO, N. F., ALMEDIA, C., CERQUEIRA, L, DIAS, S., KEEVIL, C.W., and VIERIA, M.J., 2007. Coccoid form of H. pylori as a morphological manifestation of cell adaptation to the environment.  Appl.  Envirron. Microbiol., 73: 3423- 3427. http://dx.doi.org/10.1128/AEM.00047-07

BOHMELER, G., GERWERT, I., SCUPIN, E., and SINELL, H. J., 1996.  Epidemiology of H.  pylori in human : Studies on survival capacity of  H. pylori in  food .  Dtsch. Tieraztl.  Wschenschr., 102: 438- 443. PMID: 9035978

CHONG, S.K.,  LOU, Q., FITZGERALD, J. F., and LEE, C. H., 1996.  Evaluation of 16S rRNA gene   PCR with primers Hp1 and Hp2 for detection of H. pylori.  J. Clin. Microbiol., 34: 2728– 2730. https://dx.doi.org/10.1128%2Fjcm.36.2.603-603.1998

CROWE, S. E., 2019. Helicobacter pylori infection. New England J Med., 380(12):1158–1165.

DIXIT, M. D., BHAT, K. G., and  DAYAL, A., 2012. Role of H.  pylori specific heat shock prolein -60 antibodies in the etiology of coronary artery disease.  Microbiol., Res. 3.

ELHARIRI, M., ELHELW, REHAB, HAMZA, DALIA and EL- MAHALAWY, HEBA, S., 2017. Serological evidence and risk factors for H.  pylori infection in animals and humans.  J. Infe. Dev. Ctries., 11: 414- 419. 

ELHARIRI, M., HAMZA, DALIA, ELHELW, REHAB and HAMZA, EMAN, 2018. Occurrence of cagA+ vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk. Scientific Report, 8(1).

El- SHAROUD, W. M. 2009. Prevalence and survival of Campylobacter in Egyptian dairy product. Food Research International, 42: 622- 626.DOI:10.1016/j.foodres.2009.0.009.

FAO/ WHO, 2001. Health and Nutritional Properties of Probiotics in Food Including Powder Milk and Live LAB. Food and Drug Administration/ World Health Organization. Expert Consultation Report.

FOX, J. G., CHEIN, C. C., DEWHIRST, F. E., PASTER, B. J., SHEIN, Z., MELITO, P. L., WOODWARD, D. L., and REDGERS, F. G., 2000. H. Canadensis sp. Nov. isolated from humans with diarrhea as an example of an emerging pathogen. J. Clin. Microbiol., 38: 2546- 2549. https://doi.org/10.1128/jcm.38.7.2546-2549.2000

GEORGALAKI, MARINA, K., PAPADIMITIOU, K., ANASTASIOU, RANIA, POT, B., VAN DRIESSCHE, G., DEVREESE, B., and TSAKLIDOU, E., 2013. Macedovicin, the second food-grade lantibiotic produced by streptococcus macedonicus ACA-DC 198. Food Microbiol.,33: 124- 130. http://dx.doi.org/10.1016/j.fm.2012.09.008

GOODWIN, C. S., and ARMSTRONG, J. A., 1990. Microbiological aspects of H. pylori (Campylobacter pylori). European journal of clinical microbiology, infectious disease .Official Publication of European of Clinical Microbiology, 91-13.  

GOTTELAND, M., and CRUCHET, I. A., 2003. Suppressive effect of frequent ingestion of Lactobacillus Johnson ii Lal on H.  pylori Colonization in asymptomatic volunteers.  J. Antimicrob.  Chemother., 51: 1317- 1319.

GUESSOUM, MERYEM, GUECHI, Z., and ADNANE, M., 2018. First –time serological and molecular detection of H.  pylori in milk from Algerian local-breed cows.  Veterinary world, 1326- 1330. https://doi.org/10.14202/vetworld.2018.1326-1330

KALALI, B., FORMICHELLA, L., and GERHARD, M., 2015. Diagnosis of H. pylori: changes towards the future. Diseases 3(3):122–135. https://dx.doi.org/10.3390%2Fdiseases3030122

 KAUR, L., GORDON, M., BAINES, P. A., IHEOZOR-EJIOFOR, Z., SINOPOULOU, V., and AKOBENG A. K., 2020. Probiotics for induction of remission in ulcerative colitis. Cochrane Database Syst Rev. 2020, 3:CD005573. 10.1002/14651858.CD005573.pub3

KNEIFEL, W., JAROS, D., and ERHARD F., 1993. Microflora and acidification properties of yoghurt and yoghurt- related products fermented with commercially starter cultures. Int. J. Food Microbiol., 18: 179- 189.

MADUREIRA, A. R.,   PINTADO,   M. E.,   GAMES,  A. M., and MALCATA, F. X.,  2011. Incorporation of probiotic bacteria in whey cheese decreasing the risk of microbial contamination. J. Food Prot., 47: 1194- 1199.

LAMPERT, L. M., 1975. Modern Dairy Products. 3rd ed. Chemical Pub. Co., Inc., New York.

MEGRAUD,   F., and LEHOURS,   P., 2007. H.  pylori detection and antimicrobial susceptibility testing. Clin. Micrbiol. Rev., 20: 280- 322. https://doi.org/10.1128/cmr.00033-06

MOBLEY, H. L. T., 2001. Mobley H. L. T and Mendz, G. L. and Hazell, S. I. (eds) H.  pylori: Physiology and Genetics. U. S. National library of medicine.  

MOUSAVI, M., SAFARPOOR DEHKORDI, F., and RAHIMI, F., 2014. Virulence factors and antibiotic resistance of H. pylori isolated from raw milk and unpasteurized dairy products in Iran. J.  Venomous Anim. Toxins including Trop. Dis., 20: 1-7.

OLSON, J. W., and MAIER, R. J., 2002. Molecular hydrogen as an energy Source for H. pylori. Science, 29: 1788 – 1790.

PARIKH, N. S., and AHLAWAT, R., 2021. Helicobacter pylori. In StatPearls [Internet]; StatPearls Publishing: Treasure Island, FL, USA, 2021; Available online: https://www.ncbi.nlm.nih.gov/books/NBK534233/ (accessed on 25 February 2021).

POMS,  R.  E., and TATINI, S.  R., 2001. Suvrvival of H.  pylori in ready to eat foods at 4⁰C.  Int.  J.  Food Mirobiol., 63 :281- 6.  DOI: 10.1016/s0168-1605(00)00441-4.

QUAGLIA, N. C., DAMBROSIO, A., NORMANO, G., PARISI, A., FIRINS, A., LORUSSO, V., and CELANO, G. V., 2007. Survival of H.  pylori in artificially contaminated ultrahigh temperature and pasteurized milk .  J. Food Microbiol.,  24:  296- 300. doi: 10.1016/j.fm.2006.04.008.

RANJBAR, R., FARSANI, F. Y., and DEHKORDI, F. S., 2018. Phenotypic analysis of antibiotic resistance and genotypic study of the vacA, cagA, iceA, oipA and babA genotypes of the H. pylori strains isolated from raw milk. Antimicrobial Resistance and Infection Control, 7:115 https://doi.org/10.1186/s13756-018-0409-y

SADEK, ZEINAB, I., ABOU SHADY, HALA, M., YOUSSEF, SADEK, ZEINAB, I. AND ABOU SHADY, HALA, M., YOUSSEF, and HEBAT ALLAH, I., 2011. Role of probiotic strains in the management of H. pylori. Advances in Food Science, 33: 237- 245.

SAMBASHIVAIAH S, BILICHODMATH, S., and NANJAIAH, N., 2011.  H.  pylori in periodontal pockets of chronic periodontitis patients with and without type II diabetes mellitus : a randomized controlled trial. Microbiol. Res. 2. https://doi.org/10.4081/mr.2011.e12

SAYED, AMAL, S.   M., HUSSIEN, ASMAA, A., HASSAN, MONA, A., EL-DIN, SALWA, S., AHMED, EMAN, M. M., and NOMAN, HANAN, A., 2010. A study on H.  pyloriand H.  helimanni in milk, soft cheese and the health hazard on children in Assiut Governorate . Assiut Vet.  Med. J., 56: 14- 25.

SONOMOTO, K. & A. YOKOTO, 2011. Lactic acid bacteria and Bifidobacterium: Caister Academic Press. ISBN 928. 

STEVENSON, T. H., CASTILLO, A., LUCIA, L. M., and ACUFF, G. R., 2000. Growth of H. pylori in various liquid and plating media. Appl. Microbiol., 30: 192- 196. doi: 10.1046/j.1472-765x.2000.00699.x.

SULLIVAN, A., and NORD,   C. E., 2005. Probiotics and gastrointestinal diseases.  J. Intern. Med., 257: 78-92.

TABATABAEI, M. 2012. Application of molecular and cultural methods for identification of Helicobacter spp.  In Different Animal Sources. Glob. Vet., 8: 292- 297.

TALAEI, R., SOUOD, N., MOMTAZ, H., and DABIRI, H., 2015. Milk of life stock as a possible transmission route of H.  pylori infection.  Gastroenterol.  Hepatol. Bed.  Bench., 8(1): 530-536.

TALIMKHANI, A., and MASHAK, Z., 2017. Prevalence and genotyping of H. pylori isolated from meat, milk and Vegetable in Iran. Jundishapur Journal of Microbiology, 10 (11): e14240. https://dx.doi.org/%2010.5812/jjm.14240

THABET, ASMAA, S., 2018. Occurrence of H.  pylori in Cattle,   buffaloes and patients with gastritis and peptic ulcer .  M.  S. Ss. Thesis, Fac. Vet. Med., Assiut University.

VALE, F. F., and VITOR,   J. M. .2010. Transmission pathway of H.  pylori: Dose food plays a role in rural and urban areas. Int.  J. food  Microbiol., 138:1-12. doi: 10.1016/j.ijfoodmicro.2010.01.016. Epub 2010 Jan 18.

WANG, K. Y., LI, C. S., LIU, D. S., PERNG, Y. C., SU, D. C., WU, C. M., JAN, C. H., HUANG, T. N., and WANG, W. M. 2004. Effects of ingesting Latobacillus and Bifidodacterium Containing yoghurt in subjects with Colonized H. pylori.  Am. J. Clin. Nutr., 80: 737 – 741. http://dx.doi.org/10.1093/ajcn/80.3.737

YAGHI, E., KHAMESIPOU, E.,   MASHAYEKHI, FATEMEH, M., DEHKORDI, F. S., SAKHAEI, M. H., MASOUAHDIMANESH, KHAMENEIE, M., and MARYAM, K., 2014. H.  pylori in vegetables and salads: genotyping and antimicrobial resistance properties. Biomed.  Res. Int., 757941. https://doi.org/10.1155/2014/757941

ZAMANI, M., EBRAHIMTABAR, F., ZAMANI, V., MILLER, W., ALIZADEH-NAVAEI, R., SHOKRI-SHIRVANI, J., and DERAKHSHAN, M., 2018. Systematic review with meta-analysis: the worldwide prevalence of H. pylori infection. Aliment Pharmacol Ther 47(7):868–876.

Statistics
Article View: 497
PDF Download: 411