Phytochemical Profile, Anti-lipid peroxidation and Anti-diabetic activities of Thymus algeriensis Boiss. & Reut | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 65, Issue 12, December 2022, Page 695-705 PDF (487.95 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2022.126336.5600 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Zaoui Heyem1; Boutaoui Nassima2; Menad Ahmed 3; Ramazan Erenler4; Zaiter Lahcene2; Benayache Fadila 2; Souad Ameddah  5
| ||||
| 1Laboratoire de Biologie et Environnement, Faculté de Sciences de la Nature et de la vie, Université Constantine 1, 25000 Constantine, Algérie | ||||
| 2Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Université Frères Mentouri Constantine1, Algérie | ||||
| 3Laboratory of Biology and Environment, Faculty of Nature and Life Sciences, University Mentouri Brothers Constantine 1 BP, 325 Ain El bey way, Constantine 25017-Algeria | ||||
| 4Department of Chemistry, Faculty of Arts and Sciences, Tokat Gaziosmanpaşa University, Tokat, Turkey | ||||
| 5Laboratory of Biology and Environment, Faculty of Nature and Life Sciences,University Mentouri Brothers | ||||
| Abstract | ||||
| Thymus algeriens Bioss & Reut is an Algerian native plant traditionally used for culinary and medicinal purposes. Biological activities varied with their chemical composition, which depends on the floral and geographical origin. This study was designed to screen the phenolic content of the n-BuOH extract of Thymus algeriensis (BETA), anti-lipid peroxidation and anti-diabetic properties on different in vitro models. The results showed, that BETA reduced TBARS formation in a concentration-dependent manner; protected β-carotene from auto-oxidation and prevented oxidation of ferrous in the linoleic acid/ferric thiocyanate assay. Furthermore, BETA showed effective inhibitions of the key enzymes linked to type 2 diabetes; 𝛼-amylase and 𝛼-glucosidase. BETA blocked also the formation of AGE as shown by the results of glucose-BSA (BSA-GLU), BSA-Methylglyoxal system (BSA-MGO) and glycated hemoglobin. The effectiveness of BETA in combating diabetics might be influenced by the synergistic action of its phenolic compounds; mentioned by the phenolic content and LC/MS analysis; particularly their richness on the rosmarinic acid and ferulic acid, cafeic acid as well quercetin. This suggests the potential of BETA to mitigate multiple complications stemming from oxidative stress and protein glycation in diabetes. | ||||
| Keywords | ||||
| Thymus algeriensis; LC-MS; Anti-glycation; Anti-lipid peroxidation; α-glucosidase; 𝛼-amylase | ||||
|
Statistics Article View: 578 PDF Download: 186 |
||||
