Antimicrobial and Antiproliferative activity of Tomenin compound isolated from Seriphidium Herba-Alba
El-Wassimy, M., Ahmed, M., Younes, S., Hegazy, M. (2018). Antimicrobial and Antiproliferative activity of Tomenin compound isolated from Seriphidium Herba-Alba. EKB Journal Management System, 3(1), 1-8. doi: 10.21608/sjsci.2018.233272
M. Taher M. El-Wassimy; Mehawed M. Ahmed; Sabry H. H. Younes; Mohamed-Elamir F. Hegazy. "Antimicrobial and Antiproliferative activity of Tomenin compound isolated from Seriphidium Herba-Alba". EKB Journal Management System, 3, 1, 2018, 1-8. doi: 10.21608/sjsci.2018.233272
El-Wassimy, M., Ahmed, M., Younes, S., Hegazy, M. (2018). 'Antimicrobial and Antiproliferative activity of Tomenin compound isolated from Seriphidium Herba-Alba', EKB Journal Management System, 3(1), pp. 1-8. doi: 10.21608/sjsci.2018.233272
El-Wassimy, M., Ahmed, M., Younes, S., Hegazy, M. Antimicrobial and Antiproliferative activity of Tomenin compound isolated from Seriphidium Herba-Alba. EKB Journal Management System, 2018; 3(1): 1-8. doi: 10.21608/sjsci.2018.233272

Antimicrobial and Antiproliferative activity of Tomenin compound isolated from Seriphidium Herba-Alba

Sohag Journal of Sciences
Volume 3, Issue 1, January 2018, Page 1-8  XML PDF (1.49 MB)
Document Type: Regular Articles
DOI: 10.21608/sjsci.2018.233272
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Authors
M. Taher M. El-Wassimy1; Mehawed M. Ahmed* 1; Sabry H. H. Younes1; Mohamed-Elamir F. Hegazy2
1Chemistry Department, Faculty of Science, Sohag University, Sohag 82524. Egypt
2Phytochemistry Department, National Research Centre, Giza 12622, Egypt
Abstract
Seriphidium herba-alba (Asso) Sojak (Asteraceae family) (previously named Artemisia herba-alba) is a dwarf shrub fast growing in arid and warm climates and muddy areas, used in folk medicine for the treatment of gastric disturbances such as diarrhoea, abdominal cramps and for healing external wounds. Tomenin was isolated from Seriphidium herba-alba. The structure of isolated compound was identified by the extensive spectroscopic techniques such as 1D-NMR and EI-MS. Isolated compound was subjected to antiproliferative potential against cancer cell lines of the liver (HepG-2), colon (HCT-116) and breast (MCF-7). Viability was assessed by the standard colorimetric assay using the tetrazolium salt, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT). The assay is based on the reduction of the yellow, water-soluble MTT to purple water-insoluble formazan crystals in the mitochondria of living cells. MTT assay can give a first idea of the impact of a treatment on cell viability and provides therefore general information on cytotoxicity. Additionally, the total extract of S. herba alba and isolated purified compound were tested for antimicrobial activity. Tomenin exhibited low inhibitory effect against F. solani only and all other tested micro-organisms was able to resist it. Seriphidium herba-alba total extract has good inhibitory activity against all tested pathogenic bacteria.
Keywords
Seriphidium herba-alba; Tomenin; HepG-2; HCT-116; MCF-7; MTT
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