T1107 polymer expressed potential anti-tumor effects against HepG2 cell line
Kenawy, E., Salem, M., Sanoh, N., Azaam, M. (2022). T1107 polymer expressed potential anti-tumor effects against HepG2 cell line. EKB Journal Management System, 6(1), 1-10. doi: 10.21608/jcbr.2021.92796.1227
El-Refaie Kenawy; Mohamed Labib Salem; Noura Sanoh; Mohamed Azaam. "T1107 polymer expressed potential anti-tumor effects against HepG2 cell line". EKB Journal Management System, 6, 1, 2022, 1-10. doi: 10.21608/jcbr.2021.92796.1227
Kenawy, E., Salem, M., Sanoh, N., Azaam, M. (2022). 'T1107 polymer expressed potential anti-tumor effects against HepG2 cell line', EKB Journal Management System, 6(1), pp. 1-10. doi: 10.21608/jcbr.2021.92796.1227
Kenawy, E., Salem, M., Sanoh, N., Azaam, M. T1107 polymer expressed potential anti-tumor effects against HepG2 cell line. EKB Journal Management System, 2022; 6(1): 1-10. doi: 10.21608/jcbr.2021.92796.1227

T1107 polymer expressed potential anti-tumor effects against HepG2 cell line

Egyptian Journal of Cancer and Biomedical Research
Article 1, Volume 6, Issue 1, March 2022, Page 1-10  XML PDF (1.78 MB)
Document Type: Original Article
DOI: 10.21608/jcbr.2021.92796.1227
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Authors
El-Refaie Kenawy email orcid 1; Mohamed Labib Salem2; Noura Sanoh3; Mohamed Azaam4
1Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.
2Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
3Chemistry Department, Faculty of Science, Tanta University, Egypt. Center of Excellence in Cancer Research.
4Chemistry Department, Faculty of Science, Tanta University.
Abstract
Background: Polymeric nanoparticles (NPs) are obtained naturally, semi-synthetically or synthetically by a polymerization reaction. Tetronics based ethylene oxide-propylene oxide copolymers have gained a great of interest. It demonstrated interactions with cell membranes with potential for developing new biomaterials for application in nanomedicine . Aim: The primary aim of the current study is to test the direct anticancer activity of unmodified tetronic (T1107), P-HB+aminated tetronic and N, N DMAB+aminated tetronic polymersand the associated by apoptosis and cell cycle. Materials and Methods: Synthetic polymers were prepared and characterized to confirm the modification. Anti-tumor activity was examined in vitro using human hepatocellular carcinoma (HepG2) cell line.  Cell viability (MTT), cell cycle and apoptosis were evaluated by flow cytometry. Results: Unmodified tetronic (T1107) decreased HepG2 viability (by 30%) than untreated HepG2 cells.  Treatment with P-HB+aminated tetronic and N,N DMAB+aminated did not give a significant effect on Hepg-2 cells. Treatment of HepG2 with unmodified tetronic (T1107) induced cell cycle arrest at G0 phase (39.4%), while P-HB+aminated tetronic and N,N DMAB+aminated tetronic induced cell cycle arrest at G1 phase (by 54.8% and 48.2%, respectively as compared to treatment with the doxorubicin (DOX) as a reference drug, which induced Hepg-2 cell cycle arrest at GO phase (by 39.1%). The unmodified tetronic (T1107) increased the numbers of late apoptotic cells (by 49.4%), while P-HB+aminated tetronic and N,N DMAB+aminated tetronic  did not  induced significant apoptosis. Conclusion:.Unmodified Tetronic T1107 induces an anticancer effect more than modified Tetronic polymers.  
Keywords
Apoptosis; Cancer; Cell Cycle; Tetronic; Polymer
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