Isolation and identification of pathogenic biofilm-forming bacteria invading diabetic wounds
Salah, M., Badr, G., hetta, H., Khalifa, W., Shoreit, A. (2022). Isolation and identification of pathogenic biofilm-forming bacteria invading diabetic wounds. EKB Journal Management System, 51(2), 163-178. doi: 10.21608/aunj.2022.132015.1007
Mohamed Salah; Gamal Badr; Helal hetta; Walaa Anwar Khalifa; Ahmed A. Shoreit. "Isolation and identification of pathogenic biofilm-forming bacteria invading diabetic wounds". EKB Journal Management System, 51, 2, 2022, 163-178. doi: 10.21608/aunj.2022.132015.1007
Salah, M., Badr, G., hetta, H., Khalifa, W., Shoreit, A. (2022). 'Isolation and identification of pathogenic biofilm-forming bacteria invading diabetic wounds', EKB Journal Management System, 51(2), pp. 163-178. doi: 10.21608/aunj.2022.132015.1007
Salah, M., Badr, G., hetta, H., Khalifa, W., Shoreit, A. Isolation and identification of pathogenic biofilm-forming bacteria invading diabetic wounds. EKB Journal Management System, 2022; 51(2): 163-178. doi: 10.21608/aunj.2022.132015.1007

Isolation and identification of pathogenic biofilm-forming bacteria invading diabetic wounds

Assiut University Journal of Multidisciplinary Scientific Research
Volume 51, Issue 2, May 2022, Page 163-178  XML PDF (611.26 K)
Document Type: Novel Research Articles
DOI: 10.21608/aunj.2022.132015.1007
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Authors
Mohamed Salah email orcid 1; Gamal Badrorcid 2; Helal hetta3; Walaa Anwar Khalifa4; Ahmed A. Shoreit5
1Demonstrator at Botany and microbiology department, Assiut University
2Professor of Immunology Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt
3Assistant professor of medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, 71516 Assiut, Egypt
4Assistant professor at department of Internal Medicine, Faculty of Medicine, Assiut University, 71516 Assiut, Egypt
5Professor of Microbiology, Botany and Microbiology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
Abstract
Diabetic foot ulcer (DFU) is the most serious diabetic complication. Gangrene is causing by the successive bacterial infection invading diabetic wounds and may lead to limb amputation for the diabetic patient. The bacteria inhabiting the wound exhibit the ability to forming biofilm, which is a protected mode of growth that allows cells to survive under harsh environments while also dispersing to colonize new niches. The main objective is to clarify the reasons of forming the bacterial biofilm, as well as, the multidrug resistant bacteria. The swab was used method for sample collection and bacterial isolation; different culture media as (BHI medium, MSA and TSA) were used for getting pure cultures from the pathogenic bacteria invading the diabetic wounds. Crystal violet was applied to detect the biofilm formed by these bacteria. Staphylococcus species are the most prominent bacteria invading the diabetic wounds forming a stable biofilm resistant to many antibiotics, one hundred bacterial isolated were recovered from diabetic foot ulcer belonging to five genus, namely, Staphylococcus sp., Klebsiella sp., E.coli, Pseudomonas sp., and Bacillus sp. using phenotypic analysis was confirmed by phylogenetic analysis.
Keywords
Diabetic foot ulcer; Staphylococcus species; wound infection; diabetes mellitus; Bacterial biofilm
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