GENETIC DIFFERENCES BETWEEN SUSCEPTIBLE, RESISTANT AND FIELD STRAINS OF THE COWPEA APHID, Aphis craccivora (Koch) | ||||
| Journal of Plant Protection and Pathology | ||||
| Article 2, Volume 31, Issue 6, May and June 2006, Page 3929-3937 PDF (2.66 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jppp.2006.235226 | ||||
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| Author | ||||
| Eman M. M. Radwan, | ||||
| Central Agric. Pesticides Lab., A~ric. Res. Center, Dokki Giza. | ||||
| Abstract | ||||
| Random Ampilified polymorphic. DNA Polymerase Chain Reaction (RAPD- PCR) method one from several approaches of assaying genetic variation in molecular biology science. This analysis was performed using a battery of five primers to evaluate the mutagenic effect among the cowpea aphid, Aphis craccivora resistant strain of dinotefuran insecticide (neonicotcnoid) compared with field and laboratory susceptible strains. The five primers (A-14, 16, 17, 18 and 19) yielded 96 fragments, primer A -18 detected 57 % difference between genome of field and resistant aphids. The same primer gave 33% variation between susceptible and resistant aphids genome. The highest degree of difference (60%) between the field and susceptible populations DNA sequence was revealed by primer A-14. The high diversity in genome of field and dinotefuran resistant strains compared with the susceptible strain may be explain the activity of dinotefuran against field population of cowpea aphid and . support the valuble application of this insecticide in control of A.croccivora ( Koch ). | ||||
| Keywords | ||||
| The cowpea aphid; dinotefuran (neonicotinoid insecticide); RAPD; PCR | ||||
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