PREVALENCE OF FUNGI AND TOXIGENIaTY OF A. FLAVUS AND A. OCHRACEUSISOLATES RECOVERED FROM FEEDS AND THEIR CONTROL | ||||
Egyptian Journal of Agricultural Research | ||||
Article 26, Volume 84, Issue 4, December 2006, Page 1303-1318 PDF (4.12 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejar.2006.235446 | ||||
View on SCiNiTO | ||||
Authors | ||||
RASHA H. SAYED-ELAHL1; ATEF A. HASSAN1; ABDEL-AZEM M. EL BARAWY1; RAMADAN T. M. SALEM1; WAEL M. TAWAKKOL2; HOSAM A. ABDEL-LATEIF2; MOHAMED K. REFAI3 | ||||
1Animal Health Research Institute, Agricultural Research Center, Dokki, Giza, Egypt | ||||
2Faculty of Pharmacy, Cairo University, Giza, Egypt | ||||
3Faculty of Veterinary Medicine, Cairo University, Giza, Egypt | ||||
Abstract | ||||
Two hundred samples of single feeds (yellow corn, soyabean, wheat, hay and bbin) and compound feeds (poultry ration, processed animal feeds, broiler concentrates, layers concentrates and meat and bone meal); 20 samples from each, were screened for fungal contamination. The most predominant genus was Aspergilus which was isolated from all samples. Other moulds were isolated, but in low frequency as Fenkillium sp., Filmdom sp., Cladospotium sp. and Alternaria sp.The isolated A. flavus and A. ochraceus were tested for production of aflatoxin 81 and ochratoxin A, respectively. The results of aflatoxin 81 production by A. !lavas isolated from different feeds revealed that the higher Incidence of toxigenic A. liavus was recorded from layers concentrates (50%), followed by broilers concentrates and hay(40%), whereas, A. Pavus isolated from soyabean and poultry ration samples represented low incidence (12.5% and 10%), respectively. The results of ochratoxln A production by A. ochracees Isolated from different feeds revealed that the higher incidence of toxigenic A. ochramus was reported from poultry ration (100%), followed by processed animal feeds, broilers concentrates and yellow corn (50%), whereas, the incidence of toxigenic A. ochraceus isolated from hay and layers concentrate samples showed low incidence (25%) for both. Evaluation of 2 commercial antifungals by plate assay revealed that MIC of MuvAnti mould and mycostatin was 0.75 and 1.0 pg/m1 for all fungi isolated from feeds. Following the addition of MW- Anti Mould to mouldy poultry rations, the fungal population was decreased after 2 days of treatment, and completely inactivated after 1 week. The antimycotoxin (Sat F. Dray) eliminated aflatoxin 81 and ochratoxin A, when added to contaminated feeds at a concentration between 2 and 3%. | ||||
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