NON-SURGICAL INTRAUTERINE INSEMINATION IN EWES USING FROZEN SEMEN | ||||
Journal of Animal and Poultry Production | ||||
Article 2, Volume 31, Issue 4, April 2006, Page 1933-1940 PDF (1.09 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jappmu.2006.235460 | ||||
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Authors | ||||
B. E. El-Saidy,; A. A. Sallam; M. G. Gabr | ||||
Animal Production Research Institute, Agricultural Research Center. | ||||
Abstract | ||||
This study was carried out at Sakha Research Station.Sixty six crossbred (Finn x Rahmani) ewes aging 3-5 year and weighing 50.94 kg were used to study the effect of site of semen deposition (vaginal vs. cervical) and the number of inseminated spermatozoa on conception rate. Sixty-three of them exhibited estrous after injection of 187.5 pg cloprostenol intramuscularly per ewe 9-12 days post onset of natural heat. Overall mean time at which estrous has occurred post hormonal injection was 3350:0651 hrs- and average heat duration was 46.30i1.17 hrs. They were distributed equally over 3 groups. Animals in the first group (control, group 1) were inseminated at Os cervix with a dose of 200 x 103 post thaw motile sperm. Animals in the other two groups were injected intravenously with 20 IU oxytociniewe to dilate the cervix and were intrauterine trnascervical inseminated within 8 min. of injection. Doses of 100 x 106 (group 2) and 200 x 106 post thaw motile sperm (group < /p> 3) were used for insemination. Conception rate increased as site of semen deposition was deeper in the reproductive tract. All ewes (n=21) in group one with 0.0 IU oxytocin was inseminated vaginally; their conception rate was 42.66%. Ewes in groups 2 and 3 (21 ewes each) were similar in cervical dilation, in response to oxytocin (20 IU) injection. Percentage of ewes didn't responded to oxytocin and were vaginally inseminated was 4.76% (2 ewes). They failed to conceive. Those of cervix partially dilated allowing semen deposition in the cervix (2-4 cm depth) were 9.52% (4 ewes, 2 ewes each), their conception rate was 50%, while those of cervix completely dilated and intrauterine transcervically were 35.71% (36 ewes, 16 ewes each). Their conception rates were 55.6 and 61.11%, respectively. Conception rate was significantly higher between group 3 (61.11%) and grouo 1 (42.96%). It could be concluded that non surgical intrauterine insemination in ewes using frogen thawed semen increased conception rates as site of semen deposition was deeper in the reproductive tract. | ||||
Keywords | ||||
Non-surgical; Insemination; Frozen semen; Ewes | ||||
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