PATHOGENICITY OF PHOTORHABDUS LUMINESCENS, A SYMBIOTIC BACTERIUM OF AN ENTOMOPATHOGENIC NEMATODE, AGAINST GALLERIA MELLONELLA L. AND SPODOPTERA LITTORALIS (BOISD.)

Nouh, G. M.; Hussein, M. A.

doi:10.12816/0014493

	PATHOGENICITY OF PHOTORHABDUS LUMINESCENS, A SYMBIOTIC BACTERIUM OF AN ENTOMOPATHOGENIC NEMATODE, AGAINST GALLERIA MELLONELLA L. AND SPODOPTERA LITTORALIS (BOISD.)
Egyptian Journal of Zoology
Article 7, Volume 63, Issue 63, June 2015, Page 91-98
Document Type: Original Research Papers
DOI: 10.12816/0014493
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Authors
G. M. Nouh¹; M. A. Hussein²
¹Plant Protection Research Institute, Biological Control Department, Agriculture Research Center, Giza, Egypt
²Pests and Plant Protection Department, National Research Centre, Giza, Egypt
Abstract
The Photorhabdus luminescens, a symbiotic bacterium of an entomopathogenic nematode (EPNs), Heterorhabditis bacteriophora BA1 was evaluated against greater wax moth, Galleria mellonella L. and the cotton leaf worm, Spodoptera littoralis (Boise.). P. luminescens is a gram-negative bacterium, belonging to the family Enterobacteriaceae. In this study, a fresh primary phase (Phase I) of P. luminescens was isolated from a pool of 500 infective juveniles (IJs) of H. bacteriophora and its efficiency was compared to 2-years old bacterium against 3^rdlarval instars of G. mellonella and S. littoralis under laboratory conditions. The virulence of P. luminescens to S. littoralis larvae was higher than that to G. mellonella larvae and mortality percentage increased as the concentration of (EPB) increased.The fresh bacteriumwasmore effective than 2-years old one. LC₅₀were found to be 1.31x10⁸and 1.512x10⁸ bacterial cells/ml for S. littoralis and 1.78x10⁸ and 1.95x10⁸ bacterial cells/ml for G. mellonella treated with fresh P. luminescens and 2-years old one, respectively.
Keywords
Cotton leaf worm; Entomopathogenic bacterium; Heterorhabditis bacteriophora; Honeycomb moth; pathogenicity; Septicemia; symbiosis


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