EVALUAHON AND FREEENG OF BmLY GOAT SEMEN USING UNCONVENTIONAL PACKAGING METHODS | ||||
| Journal of Animal and Poultry Production | ||||
| Article 2, Volume 31, Issue 11, November 2006, Page 6919-6929 PDF (3.52 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jappmu.2006.235641 | ||||
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| Authors | ||||
| E. l. Khalifa,1; E . S. El-Gohary1; O. A . Salama1; M. M. Awad2 | ||||
| 1Animal Production Res. lnst., Agric. Res. Center, Ministry of Agric., Egypt | ||||
| 2Animal Production Dept.. Faculty of Agric., Suez Canal Univ., lsmailia, Egypt | ||||
| Abstract | ||||
| The experimental work was conducted With two breeds of billy goats Baladi and Zaraibi to evaluate semen characteristics. compare different packaging methods and post thawing sperm recovery during incubation time. Artificial vagina was used to colect twenty ejaculates (ten pooled ejaculates from each breed) to dESign three experiments for each experimental breed The ejaculates showed progressive motility over than 80.00 % were pooled and initial pooled ejaculate characteristics were evaluated and extended with Tris-yolk-fructose (TYF) cryoprotective with 6% glycerol The first experiment was carried out with unfrozen semen to estimate initial semen characteristics (Jmotilily, live and normal) after collection, extension and equilibration period at 5‘ C for 2 hours, The second experiment was conducted with frozen semen to compare between efficiency of unconventional packaging methods (aluminum sacks and cachet tablet strips) and conventional packaging methods (straws and pellets on paraffin wax) to define the best post-thawing sperm characteristics. The third experiment was to study the effect of incubation time at 37°C to 3 hrs on posi- thawing sperm motility and sperm recovery The results recorded Significant (p < 0 01) effect between initial sperm characteristics in the first experiment the mean of progressive motility after collection, extensmn, and equilibration was 89 50 %. 88.50 °/o and 86.00 % for Baladi bucks however. for Zaraibi bucks was 8600 %, 82.50 % and 80 50 9’0, respectively. In the second and third experiments the results showed significant (p < 0.01) effect between packaging methods and incubation periods on post-thawing sperm characteristics. respectively. The post thawing sperm motility values for Baladi and Zaraibi bucks were respectively with straws (56.00%) 54.50 °/o) and aluminum sacks (55.00%. 53 50 0/0) compare to tablets (50 50 %, 47 00 %) and pellets (45 50 “/0, 42.05 %).The results showed that differences among breeds were not significant | ||||
| Keywords | ||||
| cryopreservation; packaging systems; billy goat semen | ||||
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