USING ADVANCED GENETIC TECHNIQUES IN STUDYING COTTON GENOTYPES APPLIED IN BREEDING | ||||
| Journal of Plant Production | ||||
| Article 8, Volume 31, Issue 1, January 2006, Page 193-204 PDF (3.18 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2006.235694 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| A. N. Sharaf,1; D. A. EI-Kadi2; H. F. Alatwani1 | ||||
| 1Department of Genetics, Faculty of Agriculture, Cairo UniverSity. | ||||
| 2Department of Agronomy, Faculty of Agriculture, Cairo University. | ||||
| Abstract | ||||
| Five Egyptian cotton varieties Giza 70,Giza 85, Giza 88, Giza89 and Giza 90 were crossed in complete diallele crosses meeting design and produced 20 F1s genotypes. The genetic variability and relationships among 11 cotton genotypes (five cotton varieties and six F1s) were achieved using SOS-PAGE and 10 RAPO primers. The total number of bands which produced by SOS-PAGE ranged from 17 with Giza 85 to 23 with G3 (Giza70), and the cluster analysis divided the eleven cotton genotypes into two groups. The level of polymorphism for all genotypes, as revealed by RAPO was 77.05%. In addition, RAPO analysis revealed different genetic similarity ( according to Dice coefficient). Among the 11 cotton genotypes the highest similarity was between G7 and G8. However, the G8 was 61.3% similar to its female parent G90 and 78% to its male parent G89, while the lowest genetic similarity was between G6 and G10 (54.5%). Three of five Egyptian cotton varieties were characterized with eight positive and one negative unique RAPO markers. | ||||
| Keywords | ||||
| SOS-PAGE; RAPO; Polymorphism; Genetic similarity; Positive unique bands; Negative unique bands | ||||
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