MOLECULAR DETECTION OF TOXOPLASMA GONDII IN SOME TYPES OF FISHES CAUGHT FROM THE RIVER NILE CANALS | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 4, Volume 52, Issue 1, May 2022, Page 21-28 PDF (1.13 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2022.235759 | ||||
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| Authors | ||||
| HALA M. EL-ASKARY1; REHAM K. NAHNOUSH2; MARWA A. ELMALLAWANY2; MOHAMED S. BADR3 | ||||
| 1Department of Medical Parasitology, Faculty of Medicine, Beni-Suef University3, Egypt | ||||
| 2Department of Medical Parasitology, Faculty of Medicine, Cairo University | ||||
| 3Department of Molecular Biology, Medical Research Center, Faculty of Medicine, Ain Shams University | ||||
| Abstract | ||||
| Toxoplasma parasite is one of the most successful creatures on earth being resistant to the dangers of nature and powerfully spread among a wide range of land and aquatic animals. However, the extent of aquatic spread is still not well studied. This study discovered extent of Toxoplasma parasite’s spread in fresh water fishes commonly eaten by Egyptians living in some rural areas. Quantitative real-time PCR was performed for molecular detection of Toxoplasma genomic materials within 4 types of fishes; Mugil, Atherina, Tilapia and Anguilla species. Five sites were selected within these types to be studied; intestine, gills, skin/muscle complex, brain and eye. The results showed that Toxoplasma was molecularly detected in the four types of fish with the highest percentage of infection estimated to be within gills, followed by skin/muscles complex, then intestine and eye with 0% brain infection. | ||||
| Keywords | ||||
| Toxoplasma-aquatic -waterborne; fishes-qPCR | ||||
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