MOLECULAR VARIATION FOR mtDNA HAPLOTYPES OF Drosophila melanogaster AND D. simulans NATURAL POPULATIONS BASED ON MORE ACCURATE RESTRICTION-SITE MAP | ||||
| Journal of Plant Production | ||||
| Article 13, Volume 31, Issue 6, June 2006, Page 3483-3497 PDF (4.31 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2006.236037 | ||||
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| Author | ||||
| M. S. Abdel Megeed, | ||||
| Genetics Dept., Fac. Agric., Tanta Univ., Kafr El-Sheikh, Egypt | ||||
| Abstract | ||||
| Four restriction enzymes; Hae III, Msp I (4·base cutters), Pvu II and Sail (6- base cutters), were used to reconstruct restriction-site map, with better resolution, for the mtDNA from nine geographically different natural populations of D. me/anogaster and D. simulans. A total of 32 restriction sites were scored for mtDNA haplotypes; 24 sites of them were from D. melanogaster and the remaining eight sites were from D. simulans. Five restriction sites were found to be shared between the two species. Six fragment patterns were scored for each of Hae III and Msp I and two fragment patterns were scored for each of Pvu II and Sal I. In D. simulans, only one fragment pattern was scored for each of the four restriction enzymes. Eight sites (Hae III, 0.8, Msp I, 1.0, Pvu I, 4.1, 7.1 and 11.0, and Sal 1,2.0, 7.0 and 14.8) were detected in all D. melanogaster mtDNA haplotypes and were considered to be monomorphic. The highest estimate of polymorphism (94.29%) was detected for each of the two sites of Hae III, 4.4 and 10.6. The highest significant estimates of polymorphism were recorded for each of Alexandria (0.0356::0.0012), Baltim (0.0344::0.0), sent-sueet (0.0338±0.0018), and Assiut (0.0325::0.023) haplotypes. The lowest estimate of polymorphism was recorded for Sallom haplotypes (0.1250±0.0). The highest estimate of heterozygosity (H) was recorded for Alexandria haplotype (0.0230::0.0134). The net nucleotide divergence rates (dA) ranged from 0.0014 (between the two D. simulans haplotypes) to 0.1415 (between Ismailia, D. simulans and Alexandria, D. melanogaster). The highest estimates of (dA) between D. melanogaster haplotypes were recorded between Sallom and each of Beni-Sueef (0.0509) and Assiut (0.0504). Both methods of phylogenetic tree, UPGMA and NJ joined together the .two populations of D. simulans in one cluster apart from D. me/anogaster with a branch length equal to 0.1263. The NJ tree used Assiut population as a root. There were fifty cases (10 significant and 40 highly significant) of disequilibria between the 496 combinations of the 32 scored sites with percentage of 10.08%. | ||||
| Keywords | ||||
| Molecular population genetics; molecular evolution; mitochondrial DNA; Drosophila melanogaster; D. simulans; nucleotide diversity; gametic disequilibrium; phylogenetic trees | ||||
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