BIODISTRIBUTION AND FUNCTIONAL REACTIVITY OF SOME IMMUNE CELLS IN PEYER’S PATCHES, CECAL PATCHES AND SPLEEN OF ALBINO MICE AFTER TREATMENT WITH THE NANOPARTICLES | ||||
| Egyptian Journal of Zoology | ||||
| Article 2, Volume 62, Issue 62, December 2014, Page 27-48 | ||||
| Document Type: Original Research Papers | ||||
| DOI: 10.12816/0009336 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Aziz Awaad Aziz 
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| Department of Zoology, Faculty of Science, Sohag University, Sohag, Egypt | ||||
| Abstract | ||||
| Gut-associated lymphoid tissues (GALT) including Peyer's patches (PPs) and cecal patches (CPs) have a vital role in the induction of immune response against pathogens. Spleen is considered as non-GALT; it has a variety of immune cells that support the immune response. In this study, the expression of alpha L-fucose residues and biodistribution of Lectin Ulex Europaeus Agglutinin I (UEA I+) cells, IgA+ cells, 33D1+ dendritic cells (DCs) and CD11b+ macrophage subsets in the GALT (PPs and CPs) and in non-GALT (spleen) were investigated using UEAI lectin histochemistry and immunohistochemistry. The immune response induction of alpha L-fucose residue, UEAI+ cells, IgA+ cells, 33D1+ DCs and CD11+ macrophages subsets also will be investigated after treatment with amino-conjugated fluorescent silica nanoparticles (FSNPs). After treatment with FSNPs an induction of alpha L-fucose residue was observed in the PPs M cells in all cytoplasm, rather than the apical cytoplasm as shown in the control group. Also, the number of UEAI+ cells and IgA+ cells in PPs sub-epithelial dome (SED) increased 4 times and 2 times higher, respectively, as compared with that of the control group. The CD11b+ macrophages, but not 33D1+ DCs subsets located in PPs SED, had the main role to uptake FSNPs. In the case of spleen, the number of 33D1+ DCs and CD11b+ macrophages subsets was 30 times and 25 times higher, respectively, as compared with that of control group. These data indicated that alpha L-fucose residue and IgA+ cells, 33D1+ DCs and CD11b+ macrophages subsets in PPs as GALT and spleen as non-GALT had important role, but in different ratios, in the immune system induction against FSNPs. | ||||
| Keywords | ||||
| 33D1; Alpha L-fucose; CD11b; fluorescent silica nanoparticles; IgA; Immune Response; M cells; Peyer's patches; spleen | ||||
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