ANTHER CULTURE AS A TECHNIQUE TO REGENERATE HOMOZYGOUS WHEAT (Triticum aestivum L.) GENOTYPES | ||||
Journal of Plant Production | ||||
Article 17, Volume 31, Issue 8, August 2006, Page 5081-5092 PDF (184.39 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jpp.2006.236111 | ||||
View on SCiNiTO | ||||
Authors | ||||
S. R.S. Sabry,1; O. M. EI-Shihy2; M. R.A. Nesiem2; D. S. Daghma3 | ||||
1Wheat Research Department, Field Crops Research Institute, Agricultural Research Center (ARC), Giza. | ||||
2Plant Physiology Section, Agricultural Botany Department, Faculty of Agriculture. Cairo Unvi., Giza. | ||||
3National Gene Bank and Genetic Resources (NGBGR), Ministry of Agriculture and Land Reclamation, Giza. | ||||
Abstract | ||||
Eleven wheat (Triticurn aestivum L.) genotypes were screened for salinity tolerance. Seeds were germinated under six salinity levels (0 — 12000 ppm) with quarter strength of Hogland solution. Sea salt was used as a source for salinity. Three genotypes, i.e. Sids 1, Sakha 8 and line 25 were selected for performing crosses among the salinity tolerant (Sids 1 and Sakha B) and the salinity sensitive Line 25. Three crosses were conducted between the selected genotypes and their F1 plants were grown to obtain their anthers. Anther culture was used as a breeding technique to obtain pure lines in a short time. P-48 medium was used for callus induction. Great differences were observed in callus induction among the anthers of the three crosses. Embryogenic callus induction reached 10.1, 61.9 and 51.4 % for hybrid 1 (Sids 1 x Sakha 8), hybrid 2 (Sakha 8 X Line 25), and hybrid 3 ( Sakha 8 X Line 25), reSpectively. Plant regeneration medium 190-2 was used. Frequency of regenerated plants reached 1.4, 8.5, and 9.0 % for crosses 1, 2, and 3, reSpectively. Root tips of regenerated plants were cytologically examined to determine their ploidy level. All the examined plants were haploid type. Haploid plants were treated by colchicine for chromosome doubling. The doubled haploid plants reached 22.3, 24.4, and 22.1 % for crosses 1, 2 and 3 respectively. | ||||
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