MICROTUBERIZATION OF POTATO (Solanum tuberosum L.):- 1- PROTOCOL FOR PRODUCTION OF MICROTUBER IN VITRO.
Aboshama,, H., El-Afifi, S., Abdel-kafie, O., Abd Allah, H. (2006). MICROTUBERIZATION OF POTATO (Solanum tuberosum L.):- 1- PROTOCOL FOR PRODUCTION OF MICROTUBER IN VITRO.. EKB Journal Management System, 31(9), 5909-5919. doi: 10.21608/jpp.2006.236180
H. M. S. Aboshama,; S. T. El-Afifi; Omaima M. Abdel-kafie; Hala E. Abd Allah. "MICROTUBERIZATION OF POTATO (Solanum tuberosum L.):- 1- PROTOCOL FOR PRODUCTION OF MICROTUBER IN VITRO.". EKB Journal Management System, 31, 9, 2006, 5909-5919. doi: 10.21608/jpp.2006.236180
Aboshama,, H., El-Afifi, S., Abdel-kafie, O., Abd Allah, H. (2006). 'MICROTUBERIZATION OF POTATO (Solanum tuberosum L.):- 1- PROTOCOL FOR PRODUCTION OF MICROTUBER IN VITRO.', EKB Journal Management System, 31(9), pp. 5909-5919. doi: 10.21608/jpp.2006.236180
Aboshama,, H., El-Afifi, S., Abdel-kafie, O., Abd Allah, H. MICROTUBERIZATION OF POTATO (Solanum tuberosum L.):- 1- PROTOCOL FOR PRODUCTION OF MICROTUBER IN VITRO.. EKB Journal Management System, 2006; 31(9): 5909-5919. doi: 10.21608/jpp.2006.236180

MICROTUBERIZATION OF POTATO (Solanum tuberosum L.):- 1- PROTOCOL FOR PRODUCTION OF MICROTUBER IN VITRO.

Journal of Plant Production
Article 14, Volume 31, Issue 9, September 2006, Page 5909-5919  XML PDF (175.24 K)
Document Type: Original Article
DOI: 10.21608/jpp.2006.236180
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Authors
H. M. S. Aboshama,1; S. T. El-Afifi2; Omaima M. Abdel-kafie2; Hala E. Abd Allah
1Minufiya Univ.-- Genetic Engineering and Biotechnology Res. Inst. Plant Biotechnology Depart., Egypt.
2Mans. Unlv.- Faculty of Agric., Veg. and Flor. Depart.
Abstract
This study was conducted in the tissue culture laboratory of the Horticulture
Department, Faculty of Agriculture, Mansoura University during the period from 2002
to 2005, aiming to investigate the effect of cytokinins (BAP or Kin) andror auxin (NAA)
on multiplication rate as well as auxins (2,4-D, IBA and NAA) or cytokinin (BAP) on
microtuberization of potato as a new and simple protocol for production of potato in
vitro. The effect of cytokinins and auxin on multiplication rate (shoot length, number of
both shoots and leaves per explant and fresh weight of cluster) was recorded in this
study. Shoot length was increased by all Kin levels and decreased by BAP levels
while was not affected by NAA. Moreover, either BAP or Kin increased number of
shoots per explant, especially at 0.2mgrL (4.30 and 2.77, respectively).The highest
number of leaves per explant was obtained by 0.2 mgr'L BAP (16.41) or 0.5 mglL Kin
(14.43). BAP and Kin had the same effect on increasing cluster fresh weight. The
combination between NAA combined with either BAP or Kin increased number of
leaves per explant and cluster fresh weight. BAP at (0.2 rngll. BAP + 0.1 mgfL NAA)
proved its efficiency than the similar combination of Kin and NAA (at the same
concentrations) for all studded characters.
Potato microtuberization can be done by using of any tested auxins alone
(2,4-D, IBA or NAA) each at 0.0, 0.5, 1.5 and 1.5 mgfL in MS medium supplemented
with 30gfL sucrose and incubated in dark condition. The Best results of microtuber
weight. diameter, length and numberfshoot were at 1.0 mgr'L 2,4-D (151, 5.30, 7.50
and 1.66, respectively) and at 1.5 mgfL IBA (129, 4.70, 7.50 and 1.57. respectively) or
NAA (153, 5.10, 3.20 and 1.66, respectively).
In addition, microtuberization also can be done by using BAP alone at 0.0,
1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 mgr’l. under the same condition. The Best results were at
2.0 mgfL BAP (109, 4.30, 7.70 and 2.33 for microtuber weight, diameter, length and
number/shoot. respectively).
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