MOLECULAR DIVERSITY OF FOUR EGYPTIAN WHEAT CULTIVARS REVEALED BY MICROSATELLITES. | ||||
| Journal of Plant Production | ||||
| Article 4, Volume 31, Issue 10, October 2006, Page 6275-6282 PDF (86.54 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2006.236333 | ||||
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| Authors | ||||
| O. M. El-Shihy,1; A. M. Ghallab1; H. M. Youssef lbrahim1; M. S. Roder2 | ||||
| 1Plant Physiology Dept... Faculty of Agric.. Cairo University. Egypt | ||||
| 2Leibniz Institute of Plant Genetics and Crop Plant Research (IPK). 06466 Gatersleben, Germany | ||||
| Abstract | ||||
| The objective of this study was to evaluate the genetic diversity of the most important Egyptian wheat cultivars grown in 2005 by using microsatellites (SSR) as molecular markers. The four varieties of wheat (Triticum aestivum L.) tested were Giza 168. Sids 1, Gemmeza 7 and Gemmeza 9) including Chinese Spring as standard. The 24 microsatellites used revealed a total of 93 alleles. The fragment size ranged from 75 bp in GWM3 to 265 bp in GWM931. The average number of alleles was 3.9 ranging from 2-9 alleles per locus. The average allele number was different for each cultivar. With 66 alleles the number was highest in Giza 16B, followed by 33 alleles in Bids 1. then Gemmiza 9 with 32 alleles and the lowest allele number was found in Gimmeza 7 (30 alleles). The polymorphism information content (PIC value) reflecting the gene diversity of the 24 microsatellite loci ranged from 0.46 to 0.62 with an average of 0.66. The genetic similarity level ranged from 0.22 for Chinese Spring with the Egyptian cultivars to 0.58 for Gimmeza 7 and Gimmeza 9. The correlation coefficient between PIC and number of alleles over 24 microsatellites loci was 0.62. | ||||
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